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Degradation of the Cottonseed Shell Medium and Activity Changes of Related Extracellular Enzymes From Tricholoma lobayense at Different Stages of Development(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年06期
Page:
147-149
Research Field:
Publishing date:

Info

Title:
Degradation of the Cottonseed Shell Medium and Activity Changes of Related Extracellular Enzymes From Tricholoma lobayense at Different Stages of Development
Author(s):
CHU Yang; DONG Xin-wei; NI Xin-jiang; ZHAO Jin-liang
College of Life Sciences,Yantai University,Yantai,Shandong 264005
Keywords:
extracellular enzyme; Tricholoma lobayense; substrate degradation
PACS:
S 646.1+9
DOI:
-
Abstract:
Taking Tricholoma lobayense as material,using bag cultivation method,degradation of the cottonseed shell medium and activity changes of related extracellular enzymes were determined at different stages of development from Tricholoma lobayense cultivated on the cottonseed shell medium in order to reveal the features of degradation of carbon source.The results indicated that T.lobayense had a complete set of extracellular cellulose；the activity of extracellular amylase was comparatively high at mycelial growth stage；there existed the positive correlation between the degradation rates of the cellulose and hemicelulose in the medium and the activities of extracellular carboxymenthel cellulase (CMCase),filter paper cellulase (FPase) and hemicellulase (HCase)，but no correlation was found between the degradation rates of the cellulose in the medium and the activity of β-glucosidase,no correlation was found between the degradation rates of the lignin in the medium and the activities of laccase and peroxidase；T.lobayense degraded lignin strongly,so it belongs white-rot fungi；nonlignocellulose was principally used at the stage of mycelial growth,however,lignocellulose were the main carbon source for the fruiting stage of the fungus；at the end of cultivation(107 d),the medium reduction (dry weight) was 52.40%,the respiration consumption was 43.98%,the absolute biological efficiency(biomass of fruitbodies) was 8.42%.

References:

[1]Mandels M,Hontz L,Nystrom J,et al.Enzymatic hydrolysis of waste cellulase[J].Biotechnol Bioeng,1974,16：1471-1493.

[2]王玉万,王云.构菌栽培过程中对木质纤维素的降解和几种多糖分解酶的活性变化[J].微生物学通报,1989,16(3)：137-140.

[3]Sengupta S,Sengupta S.β-Glucosidase production by the mycelial culture of the mushroom Termitomyces clypeatus[J].Enzyme and Microbial Technology,1990,12(4)：309-314.

[4]Shamala T R,Sreekantiah K R.Production of cellulases and D-xylanase by some selected fungal isolases[J].Enzyme Microb Technol,1986,8(3)：178-182.

[5]潘迎捷,陈明杰,郑海歌,等.香菇和平菇生长发育中漆酶、酪氨酸酶和纤维素酶活性的变化[J].上海农业学报,1991,7(2)：21-26.

[6]张志良,瞿伟青.植物生理学实验指导[M].2版.北京：高等教育出版社,1990.

[7]王玉万,徐文玉.木质纤维素固体基质发酵物中半纤维素、纤维素和木质素的定量分析程序[J].微生物学通报,1987,14（2）：81-84.

[8]王玉万,潘贞德,李秀玉,等.玉蕈降解木质纤维素的生理生化基础[J].真菌学报,1993,12(3)：219-225.

[9]倪新江,梁丽琨.丁立孝,等.巴西蘑菇对木质纤维素的降解与转化[J].菌物系统,2001,20（4）：526-530.

[10]倪新江,初洋,赵美,等.三个黄伞菌株不同生长发育期的几种胞外酶活性变化[J].北方园艺，2013(21)：153-156.

[11]倪新江,冯志勇,梁丽琨,等.鸡腿菇对棉子壳的降解与转化[J].微生物学通报,2002,29(2)：1-4.

[12]韩建东,宫志远,任海霞,等.利用金针菇工厂化生产的菌渣栽培洛巴伊大口蘑[J].食用菌学报,2011,18(3)：39-41.

[13]韩建东,宫志远,任鹏飞,等.金针菇菌渣栽培金顶侧耳研究[J].北方园艺,2011(21)：154-156.

[14]谢春芹,贾君,谢正林,等.金针菇菌渣栽培秀珍菇试验[J].北方园艺,2012(9)：170-172.


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Last Update: 2014-08-12