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Optimization of the Extraction Technology of Dendrobium Polysaccharides From Dendrobium officinale by Mixed Enzyme(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年06期
Page:
132-134
Research Field:
Publishing date:

Info

Title:
Optimization of the Extraction Technology of Dendrobium Polysaccharides From Dendrobium officinale by Mixed Enzyme
Author(s):
TANG ZhengCHEN Xiao-xiangHUANG Xian-zhu
School of Chemical and Biological Engineering,Hezhou University,Hezhou,Guangxi 542899
Keywords:
Dendrobium officinaleextraction technologydendrobium polysaccharidescellulaseprotopectinase
PACS:
R 931.71
DOI:
-
Abstract:
Taking Dendrobium officinale as material,by single factor experiments and orthogonal experiment with the mixed enzyme,the influences of the enzyme dosage,the composition of mixed enzyme,enzymolysis time,enzymolysis temperature,and the value of pH were adopted,the optimization extraction process of dendrobium polysaccharides from Dendrobium officinalewas studied.The results showed that the optimal extraction technology was as follow:the mixed enzyme(cellulose∶protopectinase=1∶1) concentration was 1%,the pH value was 6.5,the enzymolysis temperature was 50℃ and the extracting time was 135 min.The yield of the dendrobium polysaccharides was 41.33%.The process was reasonable with high rates of extraction of Dendrobium polysaccharides.

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Last Update: 2014-08-12