|Table of Contents|

Study on Initiation of Callus Tissues and Suspension Cell Culture of Glehnia littoralis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年06期
Page:
104-106
Research Field:
Publishing date:

Info

Title:
Study on Initiation of Callus Tissues and Suspension Cell Culture of Glehnia littoralis
Author(s):
MIAO Xiao-yan; HE Fu-qiang; ZHANG Xiao-mei
Department of Biochemistry,Baoding University,Baoding,Hebei 071000
Keywords:
Glehnia littoralis; callus tissues; cell culture
PACS:
R 282.71
DOI:
-
Abstract:
Taking leaves and stems of Glehnia littoralis as materials,MS and B5 medium were respectively employed to induce the callus of Glehnia littoralis,subculture after suspension culture,and the effects of different concentrations of carbon source,6-BA and NAA on the growth of the cells were investigated,in order to provide basis for the further establishment of Glehnia littoralis cell culture system and improved the secondary metabolite production.The results showed that the more suitable callus induction of culture medium was 1/2MS+0.4 mg/L 6-BA+1.5 mg/L NAA；the more suitable subculture medium was 1/2MS+0.2 mg/L 6-BA+1.2 mg/L NAA；and in the process of cell suspension culture,0.2 mg/L 6-BA,1.6 mg/L NAA and 20 g/L sucrose would be conducive to cell growth.

References:

[1]余椿生.北沙参[J].食品与药品,2005,7(4):43-44.

[2]龚晓健,季晖,李萍,等.沙参提取物镇咳祛痰及免疫增强作用研究[J].中国现代应用药学,2000(4)：6-8.

[3]王中民,张永祥,史美育,等.北沙参抗突变实验研究[J].上海中医药杂志,1993(5)：47-48.

[4]Matsuura H,Saxena G,Farmer S W,et a1.Antibacterial and antifungal polyine compounds from Glehnia littoralis ssp. leiocarpa[J].Planta Med,1996,62(3)：256-259.

[5]Okuyama T,Takata M,Nishino H,et a1.Studies on the antitumor-promoting activity of naturally occurring substances.II.Inhibition oftumor-promoter-enhanced phospholipid metabolism by umbelliferous materials[J].Chem Pharm Bull,1990,38(4)：1084-1086.

[6]Okuyama E,Hasegawa Z Matsushita Z,et al.Analgesic components of glehnia root(Glehnia littoralis)[J].Nat Med,1998,52(6)：491-501.

[7]张样柏,唐旭利,李国强,等.北沙参的化学成分研究[J].中国海洋大学学报,2008,38（5）:757-760.

[8]董芳,刘汉柱,孙阳,等.北沙参中佛手柑内酯的分离鉴定及体外抗肿瘤活性的初步测定[J].植物资源与环境学报,2010,9(1)：95-96.

[9]李森,蔺博超,吕平,等.珊瑚菜组织的培养及无性系的建立[J].哈尔滨师范大学自然科学学报,2008,24（5）:85-86.


Memo

Memo:
-
Last Update: 2014-08-12