Microspore Embryogenesis and Plantlet Formation of Different Genotypes of Brassica rapa ssp.pekinensis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年06期

Page:

101-104

Research Field:

Publishing date:

Info

Title:

Microspore Embryogenesis and Plantlet Formation of Different Genotypes of Brassica rapa ssp.pekinensis

Author(s):

SHI Liu¹; 2; WANG Ya-qiong¹; 2; LI Yun-long¹; 2; ZHU Zhu-jun¹; 2; ZHENG Wei-wei¹; 2; ZANG Yun-xiang¹; 2

1.The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province,Research Center of Bio-Breeding Industry,College of Agriculture and Food Science,Lin’ an,Zhejiang 311300；

2.Zhejiang Agricultural and Forestry University,Lin’ an,Zhejiang 311300

Keywords:

Brassica rapa ssp.pekinensis; microspore culture; embryoid; plantlet

PACS:

S 634.1

DOI:

-

Abstract:

Taking flower buds of 9 Brassica rapa ssp.pekinensis cultivars as materials,the effect of genotypes,6-BA and different solid medium on microspore embryogenesis were studied.The results showed that the embryogenesis rate was significantly different among cultivars.Six cultivars were induced to form embryoids and the highest yield was produced in ‘Beijing Tehaochihuoguocai’ with 12.38 embryoids per bud.Medium supplemented with 0.3 mg/L 6-BA could promote the embryogenesis rate,especially for ‘Beijing Tehaochihuoguocai’.The solid MS medium supplemented with 0.01 g/L active carbon was better than B₅ solid medium for plantlet formation from embryoids.

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