WANG Ya-jun^1,2,AN Wei¹，LUO Qing¹，MA Ping³,SHI Zhi-gang¹，ZHAO Jian-hua¹(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年05期

Page:

139-143

Research Field:

Publishing date:

Info

Title:

WANG Ya-jun^1,2,AN Wei¹，LUO Qing¹，MA Ping³,SHI Zhi-gang¹，ZHAO Jian-hua¹

Author(s):

Research on Enlargment Reproducing of AMF Microbial Inoculum and Growing Seedling Technology of Wolfberry With Mycorrhization

1.National Wolfberry Engineering Research Center,Yinchuan,Ningxia 750002；

2.Forestry College,Beijing Forestry Univerty，Beijing 100083；

3.School of Agriculture,Ningxia University,Yinchuan，Ningxia 750002

Keywords:

Ambuscular Mycorrhiza Fungus(AMF); enlargement reproducing; mycorrhization; wolfberry; growing seedling technology

PACS:

R 33

DOI:

-

Abstract:

Taking wolfberry ‘Ningqi No.7’ as tested material,4 Ambuscular Mycorrhiza Fungus(AMF) microbial inoculum had been enlarged reproduced with Trifolium pretense L.and Zae mays.With self-reproduced microbial inoculum,wolfberry seedling with mycorrhization had been reared with microbial inoculum using young-shoot-cuttage seedling technology of wolfberry.After removed from the garden，wolfberry seedling with mycorrhization were planted in test field and the field performance were investigated.It could provide theoretical reference for the application of AMF on the wolfberry and deep research.The results showed that the high quality AMF microbial inoculum could been reproduced using Trifolium pretense L.and Zae mays as host plants.The mycorrhizal fungi colonization rate was more than 94%.The spores density was from 168 to 351 per 50 g dried substrate.The survival rate of wolfberry seedling was more than 60% after inoculated by AMF microbial inoculum,and had little difference among various treatments.There were lots of fungal myceliums in the roots of wolfberry seedling inoculated by AMF microbial inoculum.The mycorrhizal fungi colonization rate of wolfberry seedling was more than 43%.The seedling height growth of wolfberry seedling with mycorrhization obviously higher than the control，and the treatment with Glomus constrictum Trappe, Glomus intraradices Schenck & Smith and mixture had extreme difference with control（P<0.01）,increased by 53.45% to 89.66% respectively.The treatment with Glomus mosseae(Nicolson & Gerdemann had striking difference with the control（P<0.05）,increased by 39.66% than that of control.The basal diameter growth higher than the control,but the LSD multi-comparative analysis showed that there no striking difference to the control.The research can offer reference for applications and deeper research of AMF on wolfberry.

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