Fermentation Condition Optimization of Endogenous Bacillus amyloliquefaciens X-278(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Fermentation Condition Optimization of Endogenous Bacillus amyloliquefaciens X-278

Author(s):: WEI Jiao-yang; FENG Long; LI Ya-ning; LIU Da-qun; CHI Guo-tong; College of Plant Protection，Agricultural University of Hebei，Biological Control Center of Plant Diseases and Plant Pests of Hebei Province，National Engineering Research Center for Agriculture in Northern Mountainous Areas，Baoding，Hebei 071001

Keywords:: endogenous Bacillus amyloliquefaciens X-278; fermentation; single factor; orthogonal experiment

Abstract:: Taking Bacillus amyloliquefaciens as test material，using single factor and orthogonal experiments，and through comparing biomass of fermentation broth and bacteriostatic activity against Verticillium dahliae Kleb，the fermentation conditions of endogenous Bacillus amyloliquefaciens X-278 were optimized.The results showed that the final optimization of the medium and conditions：1.5% glucose，2% peanut power，0.5%(NH₄)₂SO₄，0.1% CaCO₃，0.5% MgSO₄，0.5% NaCl，pH 7，culture temperature 34℃，rotation speed 180 r/min，seed liquid age was 24 h，liquid volume was 150 mL/500mL of triangular flask，inoculum size was 2%，incubation time was 72 h.After optimizing，the number of viable bacteria was to 1.5×1010 CFU/mL in the fermentation broth,and the diameter of inhabiting circle against Verticillium dahliae was 33.4 mm.

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Last Update: 2014-08-10