|Table of Contents|

Establishment of High Frequency Plant Regeneration System From the Leaf of Saussurea involucrate(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年05期
Page:
98-103
Research Field:
Publishing date:

Info

Title:
Establishment of High Frequency Plant Regeneration System From the Leaf of Saussurea involucrate
Author(s):
WEI Shan-junWU Yun-fangYANG LinLUO Yun-yanKE XiangZHOU Yi-jun
College of Life and Environmental Sciences,Minzu University of China,Beijing 100081
Keywords:
Saussurea involucrateproliferated sterile seedlingsplant regenerationhormoneinduce
PACS:
S 567
DOI:
-
Abstract:
Taking proliferated sterile seedlings of Saussurea involucrate as plant material,appropriate conditions for high frequency plant regeneration of Saussurea involucrate were investigated.The results showed that robust leaves of the seedlings could be induced to regenerate the plant in two ways.Direct regeneration way:leaf fragments in length of 3~5 mm were inoculated on the medium of MS+NAA 0.5 mg/L+6-BA 2.0 mg/L.The differentiation rate could reach (73.3±3.8)% after being cultured for 3 weeks in darkness and 4 weeks under the light.The fragments could produce healthy adventitious shoot clusters with an average number of (5.0±0.3) shoots/explant.Indirect regeneration way:calli were induced from leaf fragments inoculated on the medium of MS+NAA 0.2 mg/L+TDZ 0.5 mg/L for 3 weeks in darkness,and then were cultured on medium of MS+NAA 0.5 mg/L+6-BA 2.0 mg/L for 4 weeks under the light.(88.9±5.9)% of calli could produce adventitious shoot with an average number of (5.5±0.4) shoots/callus.After growing on the medium of MS+NAA 0.05 mg/L+6-BA 0.3 mg/L for 3 to 4 weeks,the shoots proliferated and became stronger.Thus,almost 100% of the shoots could root well on the medium of MS+IBA 0.5 mg/L+0.1%active carbon.The two protocols could be used for genetic transformation research of S.involucrate.

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Last Update: 2014-08-10