|Table of Contents|

Comparison of Extraction Methods for Capparis spinosa L.Total RNA(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年05期
Page:
91-95
Research Field:
Publishing date:

Info

Title:
Comparison of Extraction Methods for Capparis spinosa L.Total RNA
Author(s):
LUO Jiang1; YANG Li-juan1; CUI Hao-ran1; MA Chun-hui2; 3
1.College of Animal Science，Tarim University，Alar,Xinjiang 843300;
2.Key Laboratory of Tarim Animal Husbandry Science and Technology of Xinjiang Production and Construction Corps，Alar,Xinjiang 843300;
3.College of Animal Science and Technology，Shihezi University，Shihezi,Xinjiang 832000
Keywords:
Capparis spinoza L; RNA extraction; method comparison; verification
PACS:
R 282.71
DOI:
-
Abstract:
With Capparis spinosa L.as material，the method of modified SDS，RNApure Plant Plus Reagent，RNAplant Plus Reagent and RNAprep Pure Plant Kit were compared to find out the optimal method for extracting high quality total RNA from blades in wild Capparis spinosa L..The results indicated that four kinds of methods to extract total RNA were seen two electrophoretic bands of 28S and 18S，RNAprep Pure Plant Kit method to obtain an RNA bands were clear pattern，bright，stable，brightness of 28S rRNA was two times of 18S rRNA，while the value of OD260/OD280 was 2.00 and the yield of RNA was 175.3 μg/g，except RNAprep Pure Plant Kit method could be extracted from the leaves to integrity is good，high purity and high yield of total RNA，other three kinds of total RNA extraction are pollution，such as biodegradation and DNA and protein.Further using RNAprep Pure Plant Kit method from Capparis spinosa L seedlings in the whole plant，root，stem and leaf could obtain high quality total RNA，therefore，RNAprep Pure Plant Kit method was suitable for the extraction of total RNA.

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Last Update: 2014-08-10