Orthogonal Design-direct Analysis and SSR for SRAP Amplification System in Momordica charantia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年05期

Page:

85-88

Research Field:

Publishing date:

Info

Title:

Orthogonal Design-direct Analysis and SSR for SRAP Amplification System in Momordica charantia

Author(s):

LIU Meng-ya¹; 2; 3; HUANG Ru-kui²; 3; HUANG Yu-hui²; 3; HUANG Xiong-juan²; 3; CHEN Xiao-feng²; 3; FENG Cheng-cheng²

1.College of Agricultural,Guangxi University，Nanning,Guangxi 530004；

2.Vegetable Research Institute，Guangxi Academy of Agricultural Sciences，Nanning,Guangxi 530007；

3.The Key Laboratory of Crops Genetic Improvement Biological Technology of Guangxi，Nanning,Guangxi 530007

Keywords:

Momordica charantia; orthogonal design-direct analysis; SSR; SRAP

PACS:

S 642.5

DOI:

-

Abstract:

Taking Momordica charantia as test material，using SSR and orthogonal design-direct analysis to optimize the four levels of five factor(dNTP，template DNA，primer，Mg²⁺，Taq DNA polymerase).The results showed that the optimum SRAP-PCR reaction system contains 1×PCR buffer，250 μmoL/L dNTP，50 ng template DNA，1.2 μmoL/L primer，1.5 mmoL/L Mg²⁺，1.5 U Taq DNA polymerase,in a total volume of 20 μL SRAP-PCR system.

References:

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Memo

Memo:

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