In vitro Conservation Technique of Rave or Endangered Germplasm in Chirita longgangensis W.T.Wang in Guihaia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: In vitro Conservation Technique of Rave or Endangered Germplasm in Chirita longgangensis W.T.Wang in Guihaia

Author(s):: ZHANG Zhan-jiang; LI Cui; WEI Ying; HUANG Bao-you; LV Hui-zhen; Guangxi Botanical Garden of Medicinal Plants，Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement,Nanning，Guangxi 530023

Keywords:: rare or endangered; Chirita longgangensisW.T.Wang in Guihaia; germplasm resource; in vitroconservation

Abstract:: Taking Chirita longgangensis W.T.Wang in Guihaia germplasm as material，using orthogonal to research the effects of medium，growth inhibitor，phytohormone，osmotic pressure，and the growth recovery were evaluated afer in vitro conservation to establish the optimalin vitroconservation technology system of Chirita longgangensisW.T.Wang in Guihaia germplasm.The results showed that the best conservation condition in 25℃ was 1/2MS basal medium+60 g/L sucrose+4.0 g/L agar powder+5 g/L mannite supplemented+1.0 mg/L CCC，in an illuminated chambr under 12 h photoperiod of 1 600 lx light intensity，more than 50% conservation material survived after 300 days，most of them could growth well on propagation medium.The results of this study could be used for medium-term germplasm preservation in vitroof Chirita longgangensis?W.T.Wang in Guihaia.

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Last Update: 2014-08-08