|Table of Contents|

Isolation and Identification of Botrytis cinerea?in Phalaenopsis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年04期
Page:
109-111
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Botrytis cinerea?in Phalaenopsis
Author(s):
DONG Ping; ZHAO Pei-bao; REN Ai-zhi
College of Agriculture，Liaocheng University，Liaocheng，Shandong 252059
Keywords:
Phalaenopsis; Botrytis cinerea; ITS; identification
PACS:
S 682.31
DOI:
-
Abstract:
The pathogen of Botrytis cinereain Phalaenopsis?were isolated and purified，and pathogen ribosomal DNA internal transcribed spacer region were amplified and sequenced with molecular biology methods.The results showed that the sequence of ITS for the pathogen shared 99.8% homology with that of Botryotinia fuckeliana?and Botrytis cinerea?in GenBank.Combined with morphological characteristics，the pathogen was identified as Botryotinia fuckeliana，anamorphic was Botrytis cinerea.

References:

[1]谢启鑫,缪南生,宋小民,等.蝴蝶兰种质资源遗传多样性的ISSR分析[J].西北植物学报,2010,30(7):1331-1336.

[2]胡松华.热带兰花[M].北京:中国林业出版社,2002:45-46.

[3]谭巍.蝴蝶兰常见真菌、细菌性病害及防治措施[J].北方园艺,2006(2):128-129.

[4]易绮斐,刘东明,陈红峰,等.兰花主要病害及其防治[J].植物保护,2004(1):71-72.

[5]孟祥东,傅俊范,周如军,等.保护地主要园艺作物灰霉病菌生物学特性比较研究[J].沈阳农业大学学报,2007,38(3):322-326.

[6]Celik M.Preparation and characterization of starchg poly methacrylamide copolymers[J].J Polym Res,2006,13(5):427-432.

[7]周德庆,祖若夫.真菌单孢子分离的一种简易方法[J].微生物通报,1979,6(2):35-36.

[8]Hirata T，Cunnington J H，Paksiri U，et al.Evolutionary analysis of subsection Magnocellular of Podosphaerasection Sphaerotheca(Erysiphales) based on the rDNA internal transcribed spacer sequences with special reference to host plants[J].Can J Bot,2000,78:1521-1530.

[9]徐森富.番茄灰霉病的发生与防治措施[J].中国园艺文摘,2011(6):128-129.

[10]黄亚丽,王淑霞,杜晓哲,等.一株具有诱导抗性木霉菌株的筛选及其对黄瓜灰霉病诱导抗性的初步研究[J].植物保护,2013,39(1):38-43.

[11]刘耕春,程霞英,王万立,等.花卉灰霉病病原及发病特点[J].天津农业科学,2004(2):44-46.

[12]张宏伟,顾俊杰,石达祺.东方百合灰霉病防治[J].中国花卉园艺，2009(24):34-35.

[13]瞿友均,徐源辉.百合灰霉病的发生特点和综合防治方法[J].中国植保导刊，2010(4):26-27.

[14]易绮斐,刘东明,陈红锋,等.兰花主要病害及其防治[J].植物保护，2004(1):71-73.


Memo

Memo:
-
Last Update: 2014-08-08