Study on Lily Distant Hybridization in vitroCulture of Immature Ovary(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Lily Distant Hybridization in vitroCulture of Immature Ovary

Author(s):: ZHAO Xing-hua; WU Hai-hong; YANG Jia-ming; Horticulture Branch，Liaoning Academy of Agriculture Sciences，Shenyang，Liaoning 110161

Abstract:: Taking different inter specific hybridization obtained lily ovary as explants，according to the degree of development of the ovary，the slice using ovary，ovules or small embryos were cultured in vitroto observe sliced ovary，ovules and small embryo germination and growth，and filter out suitable germination and growth medium for small embryo，ovule and ovary slices.The results showed that good ovary slice culture medium was MS+NAA 0~0.3 mg/L+sucrose 30 000 mg/L；suitable ovule culture medium was 1/2 MS+BA 0~0.2 mg/L+sucrose 30 000 mg/L；suitable for small embryo culture medium was 1/2 MS+BA 0~0.2 mg/L+NAA 0~0.2 mg/L+sucrose 30 000 mg/L.

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Last Update: 2014-08-08