|Table of Contents|

Establishment of Efficient Plantlet Regeneration System From Petiole Explants for Brassica pekinensiscv.‘Luoyang baotou’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年04期
Page:
86-89
Research Field:
Publishing date:

Info

Title:
Establishment of Efficient Plantlet Regeneration System From Petiole Explants for Brassica pekinensiscv.‘Luoyang baotou’
Author(s):
DU Li; YAO Yao; LI Yong-peng
School of Life Science and Technology，Nanyang Normal University，Nanyang，Henan 473000
Keywords:
Brassica pekinensiscv.‘Luoyang baotou’; petiole; tissue culture; plantlet regeneration
PACS:
S 634.1
DOI:
-
Abstract:
Taking the no vaccine petiole of Brassica pekinensiscv.‘Luoyang baotou’ as explant，the cabbage plant regeneration system was established by direct organogenesis.The results showed that the effects of different plant hormones combinations at different concentrations on seedlings propagation，adventitious buds induction from petiole explants and seedling rooting were different.The optimal medium for propagation of seedlings was MS supplemented with BA 1.0 mg/L+IBA 0.3 mg/L，and the induction rate was 86.7%；the highest adventitious buds induction frequency was 76.7% by using MS supplemented with BA 2.5 mg/L+IBA 0.3 mg/L，the optimal medium for rooting was MS supplemented with IBA 1.0 mg/L and the rooting rate was 90.0%.

References:

[1]吴玲玲,王一娟,董刚,等.大白菜再生体系的正交设计优化[J].山西大学学报(自然科学版),2012(4):712-716.

[2]孟凡珍,张振贤,于贤昌.不同生态型结球大白菜抗寒性的评价[J].中国农业大学学报,2004(4):35-39.

[3]Jaglo K R，Klef S，Amundsen K L，et al.Components of the ArabidopsisC-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napusand other species[J].Plant Physiol,2001,127:910-917.

[4]Hsieh T H，Lee J T，Yang P T，et al.Heterology expression of the?ArabidopsisC-repeat/dehydration response element binding factor 1 gene elevated tolerance to chilling and oxidative stresses transgenic tomato[J].Plant Physiol,2002，129:1086-1094.

[5]Hsieh T H，Lee J T，Chang Y Y，et al.Tomato plants ectopically expression ArabidopsisCBF1 show enhanced resistance to water deficit stress[J].Plant Physiol,2002,130:618-626.

[6]孟平红,万发香,王永清,等.冷诱导转录因子CBF3转化茄子的初步研究[J].中国蔬菜,2013(10):36-43.

[7]刘任源,于拴仓,魏佑营,等.白菜类作物子叶高频再生体系的建立[J].西北农业学报,2012(6):118-123.

[8]王洋,崔继哲,李翠.大白菜高频再生体系的建立及策略[J].园艺学报,2005,32(4)：701-703.

[9]张松,温孚江,魏毓棠.外植体处理及接种方式对大白菜植株再生的影响[J].山东农业大学学报(自然科学版),2001(1):78-80.

[10]郝晓云,沈海涛,李鸿彬.甘蓝型油菜下胚轴和带柄子叶再生体系研究[J].生物技术通报,2013(4):69-74.

[11]杨长友,袁中厚,郑小敏,等.甘蓝型油菜高效离体再生体系的建立[J].生物技术通报,2013(1):111-115.

[12]刘学成,张鲁刚,茹磊,等.大白菜真叶高频再生体系的建立[J].西北农林科技大学学报(自然科学版),2011(11):146-152.

[13]郭艳超,张倩,田传卫,等.香水月季类原球茎(PLBs)途径再生植株的研究[J].中国农业大学学报,2008(5):29-34.

[14]田琳琳,赵梁军,张睿,等.无刺桅杆槐和无刺槐再生体系的研究[J].中国农业大学学报,2010(4):39-44.

[15]徐茜,乐正碧,徐燕,等.甘薯叶片和叶柄组织诱导培养及植株再生研究[J].中国农学通报,2011(15):102-105.

[16]张朝军,范术丽,武芝霞,等.棉花大田植株叶柄组织培养体系的建立[J].西北植物学报,2011(6):1257-1263.

[17]曹家树,余小林,黄爱军,等.提高白菜离体培养植株再生频率的研究[J].园艺学报,2000(6):452-454.

[18]史超,王银娟,杜丽,等.大白菜“早熟5号”不定芽再生体系的优化[J].江苏农业科学,2011(5):71-73.


Memo

Memo:
-
Last Update: 2014-08-08