|Table of Contents|

Effect of Salt Stress on Expression of Lycopene Cyclase Gene in Processing Tomato Leaves(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年04期
Page:
82-85
Research Field:
Publishing date:

Info

Title:
Effect of Salt Stress on Expression of Lycopene Cyclase Gene in Processing Tomato Leaves
Author(s):
ZHANG Fen1ZHANG Bo2TIAN Li-ping12XUE Lin3LI Hui-ling1ZENG Yi-hui3
1.College of Life Science, Shihezi University,Shihezi,Xinjiang 832000;
2.College of Pharmacy,Shihezi University,Shihezi,Xinjiang 832000;
3.Vegetable Research Institute of Shihezi,Shihezi,Xinjiang 832000
Keywords:
salt stressprocessing tomatolycopene cyclase(Lyc-β)expression
PACS:
S 641.2
DOI:
-
Abstract:
Taking three tomato cultivars ‘Shihong305’,‘Ligeer 87-5’ and ‘LA2711’ as material,the expression of lycopene cyclase gene (Lyc-β) of processing tomato leaves under the conditions of different salinity level was checked using semi-quantitativeRT-PCR,in order to study the molecular mechanism of carotenoids synthesis in processing tomato leaves under salt stress.The results showed that the expression of Lyc-β gene could be up or down regulated at the certain range of salinity,it also found that different cultivars changed differently when the salinity level increased,which could be related to the tomato’s genotype and their ability of salt tolerance.HPLC was used for determination of β-carotene content in corresponding leaves,the results illustrated that the change of Lyc-β gene expression was correlated with the change of β-carotene content in the leaves.This study further revealed that salt stress could affect the metabolism of β-carotene synthesis by regulating the expression of lycopene cyclase gene.

References:

[1]Parida A KDas A B.Salt tolerance and salinity effects on plants:a review[J].Ecotoxicology and Environmental Safety200560324-349.

[2]杨式华,徐济仓,盛冀萍,.烟草中质体色素研究进展[J].云南化工,200734(1)69-73.

[3]贾峰,徐文,刘卫群,.温度与光照强度对烤烟番茄红素β-环化酶基因表达的影响[J].植物生理学报,201147(2)189-192.

[4]Dharmapuri SRosati CPallara Pet al.Metabolic engineering of xanthophyll content in tomato fruits[J].FEBS Lett2002519(1)3034.

[5]刘蕾.新疆土壤盐胁迫的组成和分布特征[J].干旱环境监测,200923(4)227-229.

[6]中国无土栽培技术论坛.常用无土栽培营养液配方[EB/OL].http//www.soilles.com2012-11-7.

[7]李莉,侯丽霞,施木田,.番茄红素环化酶基因片段克隆及反义表达载体构建[J].山东农业科学,2009(1)1-7.

[8]刘国顺,韦凤杰,王芳,.反相高效液相色谱法测定烤烟叶片发育过程中的类胡萝卜素类物质[J].色谱,200624(2):161-163.

[9]王新超,陈亮,赵丽萍,.反相高效液相色谱法测定茶叶中β-胡萝卜素含量[J].中国农学通报,2006,22(9)91-93.

[10]Bramley P M.Regulation of carotenoid formation during tomato fruit ripening and Development[J].Journal of Experimental Botany20023772107-2113.

[11]Rodrigo M JMarcos J FZacarias L.Biochemical and molecular analysis of carotenoid biosynthesis inflavedo of orange(Citrus sinensis L.) during fruit development and maturation[J].Agric Food Chem200452(22):6724-6731.

[12]陈义强,刘国顺,凌爱芬.氮、磷、钾肥和土壤水分对烟草烤后叶中类胡萝卜素含量的影响[J].植物生理学通讯,200844(2)279-282.

[13]曹勇,管安伟,谢强,等.土壤质地对烤烟叶片类胡萝卜素及叶绿素的影响[J].江西农业学报,201224(6):130-134.

[14]白森,韦建玉,邓宾玲,等.海拔高度对烟叶多酚和类胡萝卜素含量的影响[J].贵州农业科学,201240(7)66-68.

[15]朱长甫,陈星,王英典.植物类胡萝卜素生物合成及其相关基因在基因工程中的应用[J].植物生理与分子生物学学报,2004306):609-618.

[16]牛艳,许兴,魏玉清,等.不同产地土壤因子与宁夏枸杞中β-胡萝卜素关系的研究[J].农业科学研究,200526(2)21-39.


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Last Update: 2014-08-08