Isolation and Fungicide Screening of Cucumber Fusarium Wilt(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: WU Yu-huan¹; LU Jian-bin²; YU Wen-bin³; WU Ya-ming¹; ZHANG Hong-jie¹; 1.Hebei North University，Zhangjiakou，Hebei 075000；
2.Agricultural Information Websites of Zhangjiakou，Zhangjiakou，Hebei 075000；
.Zhangjiakou Academy of Agricultural Sciences，Zhangjiakou，Hebei 075000

Abstract:: Taking cucumber disease plants of fusarium wilt as material，the pathogen was isolated through tissue isolation and pathogen morphological observation of cucumber was done，and toxicity tests of twelve fungicides to Fusarium oxysporum were carried out using inhibiting growth rate in laboratory，and the effect of seven different kinds of chemical agents on control cucumber fusarium wilt was investigated by potted plant trial.The results showed that the isolate was identified as Fusarium oxysporum.The effect of 36% evil mildew ? thiram WP 1 000 times liquid was the best with control effect of 93.92%，followed by 10% difenoconazole WG 1 500 times liquid，62.5% fine fludioxonil FS 1 500 times liquid and 99% hymexazol powder 3 000 times liquid with control effect 85.48%，81.20% and 80.45% respectively.80% pyrimethanil WDG，99% hymexazol powder and 32.5% benzoyl?azoxystrobin SC were good fungicides for control cucumber fusarium wilt，and the control effect was between 79.48%~93.61%.These fungicides could be used as a soil treatment agent on cucumber fusarium wilt，and they were safe to cucumber，so they should be widely exploited and extended in the future.

[1] 董金皋 . 农业植物病理学 [M].2 版 . 北京 : 中国农业出版社 ,2007. 

[2]顾明洁.几种杀菌剂对黄瓜枯萎病菌的联合毒力[J].西北农业学报,2008,17(1):98-101.

[3]李春芝.瓜类枯萎病药剂防治研究[J].现代农业科技,2010(2):185,189.

[4]陆家云.植物病原真菌学[M].北京:中国农业出版社,2001.

[5]阮华芳,蒙美莲.黄瓜枯萎病原镰刀菌的鉴定[J].内蒙古农牧学院学报,1987,8(2):139-144.

[6]陈霞,刘东,张艳菊,等.黄瓜枯萎病病株镰孢菌的分离与鉴定[J].东北农业大学学报,2010,41(7):37-44.

[7]程智慧,宋莉,孟焕文.大蒜鳞茎粗提物对黄瓜枯萎病的抑菌作用和防病效果[J].西北农林科技大学学报,2008,36(5):113-118.

[8]刘金秀,马正,申屠旭萍,等.黄瓜枯萎病拮抗放线菌筛选及其生防作用鉴定[J].园艺学报,2012,39(6):1123-1130.

[9]马丽娟,周宝利,张淑红,等.蛇床子提取物对黄瓜枯萎病菌抑制效果的研究[J].植物保护,2008,34(4):36-39.

[10]赵飞龙,董海龙,仲嘉伟,等.三种植物提取物对黄瓜枯萎病菌的离体抑菌活性测定[J].山西农业科学,2009,37(2):50-52.

[11]苗则彦,赵奎华,刘长远,等.内生细菌B504的鉴定及对黄瓜枯萎病的生防作用[J].植物保护,2009,35(6):73-77.

[12]郎剑锋,刘鸣韬,田雪亮,等.六种植物源活性物质对黄瓜枯萎病菌的抑菌作用[J].湖北农业科学,2009,48(12):3030-3032.

[13]赵帅,田长彦,史应武,等.黄瓜枯萎病生防菌HD-087产抗菌物质条件的优化及抑菌作用初探[J].微生物学通报,2013,40(5):802-811.

[14]周红梅,毛爱军,张丽蓉,等.黄瓜枯萎病接种方法及抗性遗传的研究[J].华北农学报,2010,25(4):186-190.

[15]段广荣,石延霞,谢学文,等.黄瓜枯萎病防治药剂的离体和活体筛选[J].中国蔬菜,2010(12):60-65.

[16]陈炳旭,黄汉杰.30%恶霉灵水剂防治瓜类病害的药效试验[J].农药,1998,37(7):38-39.

[17]肖敏,吉训聪,陈昶.3%恶霉灵·甲霜灵水剂防治黄瓜枯萎病[J].植物保护,2005,31(5):87-88.

Last Update: 2014-08-22