Study on Tissue Culture and Rapid Propagation on Stem Tip of Rosa damascus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Tissue Culture and Rapid Propagation on Stem Tip of Rosa damascus

Author(s):: HUANG Ying¹; HU Lei²; GU Qing¹; WANG Dong-liang¹; 1.College of Horticulture, Anhui Agricultural University，Anhui，Hefei 230036;
2.Anhui Occupational College of City Management，Anhui，Hefei 230041

Abstract:: Taking stem tip of Rosa damascena as the explant,different kinds and different concentration of plant hormones in MS that influenced stem tip germination,adventitious bud proliferation and adventitious root were studied.The results showed that exogenous plant hormones 6-BA and NAA played an important role in fostering stem tip germination，and 6-BA 1.0 mg/L+NAA 0.1 mg/L was the optimum combination，induction rate reached 93.3%;during the subculture,the optimum subculture medium proliferation was MS+6-BA 1.0 mg/L+NAA 0.05 mg/L;and the percentage of rooting medium was the highest containing 1/2MS+IBA 0.5 mg/L+0.3% activated carbon+0.7% agar+3% sugar.

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Last Update: 2014-08-22