|Table of Contents|

Essential Characteristics and Expression Analysis of Two RNA Helicase Genes in Apple(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年03期
Page:
91-96
Research Field:
Publishing date:

Info

Title:
Essential Characteristics and Expression Analysis of Two RNA Helicase Genes in Apple
Author(s):
XU Rui-rui1ZHANG Shi-zhong2CAO Hui1LIU Hui-lian1WANG Jian-bo1
1.Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong,Weifang University,Weifang,Shandong 261061;
2.National Research Center for Apple Engineering and Technology,Shandong Agricultural University,Tai’an,Shandong 271018
Keywords:
Malus×domesticaRNA helicasegene characterizationpromoter sequence analysisexpression analysis
PACS:
S 661.1
DOI:
-
Abstract:
The chromosome location,basic characteristics of amino acids,phylogenetic analysis,promoter elements of two Mdhelicase genes were predicted and analyzed with bioinformatics methods gene expression difference at fruit maturity and rootstockscion interactions were analyzed;with plant CARE database,responsive elements included in promoter seguence of two Mdhelicase gene were predicted and analyzed;the expression of Mdhelicase gene was analyzed in two RNA helicase gene in apple with guantitative RT-PCR.The results showed that two Mdhelicase genes from ‘Jinguan’ apple,called Mdhelicase1 and Mdhelicase2,were mapped on chromosomes 6,17,respectively.Microarray analysis showed that the expression levels of the Mdhelicase1 and Mdhelicase2,were altered during the ripening process and rootstock-scion interactions process.Using the PlantCARE database,several known stress responsive elements were identified,such as MBS,HSEs,TC-rich repeat,AREs,ABREs,GARE motif,P box,CGTCA motif,TGACG motif,Light responsive elements,etc.Expression analysis showed that two Mdhelicase genes expressed in stem,leaf,flower and fruit,with the highest expression level in stem and leaf,respectively.

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Last Update: 2014-08-22