|Table of Contents|

Effect of Citrus grandis var.shatinyu Hort.Style Protein on Its Pollen Germination and Growth(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年03期
Page:
88-91
Research Field:
Publishing date:

Info

Title:
Effect of Citrus grandis var.shatinyu Hort.Style Protein on Its Pollen Germination and Growth
Author(s):
QIN Ping-shengZHANG YuHOU Li-xiaYANG Xiao-wenQIN Xin-min
College of Life Science,Guangxi Normal University,Guilin,Guangxi 541004
Keywords:
Citrus grandis var.shatinyu Hort.pollen germinationpollen tube lengthstyle proteinsGel electrophoresis
PACS:
S 666.3
DOI:
-
Abstract:
Taking the pollen of Citrus grandis var.shatinyu Hort. as material,effect of the self-pollinated style proteins and the cross-pollinated style proteins on pollen germination and pollen tube growth were compared.The results showed that pollen germination was unaffected by adding self-pollinated style protein and the cross-pollinated style protein,but significantly affected the growth of pollen tube.The growth of pollen tube in those media with the self-pollinated style proteins was restrained,with cross-pollinated style proteins was unaffected.PAGE electrophoresis showed that the pollen tube self style protein processing of the 1 specific protein bands (Rf=0.81);tube SDS-PAGE electrophoresis to self styleprotein processing appeared relative molecular weight of 2 specific protein of 35 kD and 37.5 kD band;IEF-PAGE electrophoresis results showed that,in the self style protein treated pollen tube protein in the isoelectric points of 5.85 and 6.20 of the two specificprotein bands.

References:

[1] 薛妙男 , 陈腾土 , 杨继华 . 沙田柚自交和异交亲和性观察 [J]. 园艺学报, 1995,22(2):127-132.

[2]薛妙男,杨继华,张杏辉.沙田柚自交不亲和性结构基础研究[J].植物学通报,1996,13():87-88.

[3]薛妙男,杨继华.沙田柚花粉管在花柱中的生长途径及其识别[J].广西师范大学学报(自然科学版),2001,19(2):60-66.

[4]薛妙男,李义平,张杏辉,.沙田柚自交花柱S1-RNase的免疫胶体金定位[J].广西师范大学学报(自然科学版),2000,18(1):81-84.

[5]杨继华,尧桂荣,薛妙男.沙田柚花柱S-糖蛋白的纯化和N-端序列测定[J].广西师范大学学报(自然科学版),2001,19(1):72-79.

[6]杨继华,颜承,薛妙男,等.沙田柚花粉蛋白质双向电泳分析[J].广西师范大学学报(自然科学版),2002,20(2):73-77.

[7]Brewbaker J L,Kwack B H.The essential role of calcium ion in pollen germination and pollen tube growth[J].Am J Bot1963,50:859-865.

[8]郭尧君.蛋白质电泳实验技术[M].北京:科学出版社,2005:42-157.

[9]Vesterbery O.Isoelectric focusing of protein[J].Metheds in Enaymology,1971(22):389-412.

[10]向旭,欧喜良,邱燕萍,等.影响荔枝花粉活力的化学因子研究[J].广东农业科学,2000(6):29-32.

[11]Vaknin YEisikowitch D.Effects of short-term storage on germinability of pistachio pollen[J].Plant Breeding2000119(4):347-350.

[12]杨继华,李红艳,薛妙男.沙田柚花柱S-糖蛋白的分离与鉴定[J].广西师范大学学报(自然科学版),2000,18(4):66-70.

[13]Anderson M A,Cornish E C,Mau S L,et al.Cloning of a cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata[J].Nature,1986,321:38-44.

[14]Bredemeijer G M MBlass J.S-specific proteins in style of self-incompatible Nicotiana alata[J].Theo Appl Genet1981,59:185-190.

[15]Huang SLee H SKarunanandaa Bet al.Ribonuclease activity of Petunia injlata S-proteins is essential for rejection of self-pollen[J].Plant Cell,1994(6)1021-1028.

[16]Royo JKuntz CKowyama Yet al.Loss of a histidine residue at the active site of S-locus ribonuclease is associated with self-compatibility in Lycopersicon peruvianum[J].Proc Natl Acad Sci USA,199491:6511-6514.

[17]Certal A CSanchez A MKokko Het al.S-RNases in apple are expressed in the pistil along the pollen tube growth path[J].Sexual Plant Reproduction,1999,12(2):94-98.


Memo

Memo:
-
Last Update: 2014-08-22