Comparison Study on Pollen Viability Determining Methods of Citrus reticulate ‘Shatangju’ in Solar Greenhouse(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Comparison Study on Pollen Viability Determining Methods of Citrus reticulate ‘Shatangju’ in Solar Greenhouse

Author(s):: GAO Xing-ying¹; CUI Shi-mao¹; FU Chong-yi²; LIU Wei¹; CHU Yi-hong¹; ZHU Shan-chuan¹; 1.College of Agronomy，Inner Mongolia Agricultural University，Hohhot，Inner Mongolia 010019；
2.Vegetable Institute，Inner Mongolia Academy of Agriculture and Animal Husbandry，Hohhot，Inner Mongolia 010031

Keywords:: Citrus reticulate ‘Shatangju’; pollen viability; in vitro pollen germination method; MTT staining method; TTC staining method; I₂-KI staining method

Abstract:: Taking grafted seedings of Citrus reticulate ‘Shatangju’ on rootstock of trifoliate orange as material，the determination effects of I₂-KI,TTC,MTT and in vitro pollen germination method were compared and their effect on in vitro activity of pollen between the different types of flower(the flower with leaves and non-leaf flower) of Citrus reticulate ‘Shatangju’ were studied.The results indicated that the pollen of Citrus reticulate ‘Shatangju’ viability of non-leaf flower was higher than the pollen viability of flower with leaves.MTT staining method was the optimum and rapid staining method to determine pollen viability of Citrus reticulate ‘Shatangju’.

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Last Update: 2014-08-21