|Table of Contents|

Optimization for SCoT-PCR System and Primer Selection of Rosa rugosa(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年23期
Page:
91-96
Research Field:
Publishing date:

Info

Title:
Optimization for SCoT-PCR System and Primer Selection of Rosa rugosa
Author(s):
WANG Yu1; ZHAO Lan-yong1; SHEN Hong-wei2; QI Yu1; SHI Liang-hong1; WU Di1
1.College of Forestry,Shandong Agricultural University,Tai’an,Shandong 271018;
2.Garden Virescence and Maintenance Center of Rizhao,Rizhao,Shandong 276800
Keywords:
Rosa rugosa; SCoT-PCR; orthogonal design; single factor experiment; system optimization; primer screening
PACS:
S 685.12
DOI:
-
Abstract:
Taking Rosa rugosa as material，L16(45) orthogonal design test and single factor experiment were applied for optimizating five factors (DNA template,Mg2+,dNTPs,primer and TaqDNA polymerase) in the SCoT-PCR reaction system of Rosa rugosa,and primers were screened in order to establish the best reaction system of Rosa rugosa.The results showed that the best SCoT-PCR system for Rosa rugosa included 1.5 ng/μL DNA，2.00 mmol/L Mg2+，0.35 mmol/L dNTPs，0.70 μmol/L primer,and 0.50 U Taq DNA polymerase and 20 primers were screened which had abundant and clear polymorphism bands.The optimized SCoT-PCR system and the screeTaking Rosa rugosa as material，L16(45) orthogonal design test and single factor experiment were applied for optimizating five factors (DNA template,Mg2+,dNTPs,primer and Taq DNA polymerase) in the SCoT-PCR reaction system of Rosa rugosa,and primers were screened in order to establish the best reaction system of Rosa rugosa.The results showed that the best SCoT-PCR system for Rosa rugosa included 1.5 ng/μL DNA，2.00 mmol/L Mg2+，0.35 mmol/L dNTPs，0.70 μmol/L primer,and 0.50 U TaqDNA polymerase and 20 primers were screened which had abundant and clear polymorphism bands.The optimized SCoT-PCR system and the screened primers provided a theoretical basis and technical support for the further study on Rosa rugosa with SCoT-PCR.ned primers provided a theoretical basis and technical support for the further study on Rosa rugosa with SCoT-PCR

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Last Update: 2014-12-23