Research on Differentiation and Plant from in vitro Leaf Tissues of Chirita heterotricha Merr.O(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Research on Differentiation and Plant from in vitro Leaf Tissues of Chirita heterotricha Merr.O

Author(s):: PAN Mei; QI Hua-sha; HUANG Sai; WANG Jing-fei; FU Rui-kan ; YUN Yong; PAN Mei，QI Hua-sha，HUANG Sai,WANG Jing-fei,FU Rui-kan ,YUN Yong

Abstract:: Using the leaves of Chirita heterotricha as explants，MS as basic culture medium，the effect of plant growth regulators on adventitious bud induction，multiplication and root formation were studied in order to establish regeneration reproductive system of Chirita heterotricha Merr.O.The results showed that using the MS+6-BA 0.5 mg/L+NAA 0.1 mg/L medium，adventitious bud differentiation rate reached 100%;the medium on shoot proliferation and growth was good，40 days multiplication factor reached 7.43;MS+NAA 0.5 mg/L medium，30 days rootage rate was 100%;the rooted plantlets were transplanted in the pearlite matix and the survival rate was over 94%.

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Last Update: 2014-12-23