Study on Rapid-Micropropagation Technique by Shoot Tip Culture of New Apple Dwarf Rootstock Strain ‘Short Stock No.6’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Rapid-Micropropagation Technique by Shoot Tip Culture of New Apple Dwarf Rootstock Strain ‘Short Stock No.6’

Author(s):: WANG Miao-miao; MA Xiao-yue; ZHANG Xue-ying; XU Ji-zhong; College of Horticultural,Hebei Agricultural University,Baoding,Hebei 071001

Keywords:: apple; dwarf rootstock; shoot tip culture; rapid-micropropagation; plant growth regulator

Abstract:: Taking the new strain ‘Short Stock No.6’ of apple dwarf rootstock as materials,the effects of plant growth regulators on multiplication and rooting of seedlings in vitro were researched to selecte the optimal medium.The results showed that the sterilization time of shoot tip using 0.1% HgCl2 was 8 minutes.The basic medium was MS supplemented with sugar 30 g/L and agar 6 g/L,the optimal plant growth regulators in subculture medium were 6-BA 1.0 mg/L+NAA 0.05-0.10 mg/L,and the coefficient of multiplication reached 4.53 times every four weeks.The optimal plant growth regulators of subculture medium that suited rooting were 6-BA 1.0 mg/L+NAA 0.1 mg/L,the optimal plant growth regulators for rooting were IAA 1.0 mg/L+IBA 0.4 mg/L,the rooting rate was more than 90%,and the average number of root was about 6.Then the rooted plantlets were transplanted,the survival rate was more than 90%.

[1]韩振海.苹果矮化密植栽培——理论与实践[M].北京:科学出版社,2011.

[2]刘国荣,陈海江,徐继忠,等.矮化中间砧对红富士苹果果实品质的影响[J].河北农业大学学报,2007,30(4):24-26

[3]宋春河,张明勇,宋秀玲,等.矮化中间砧长富2苹果树早期丰产技术[J].中国果树,1999(4):42-44.

[4]原永兵,刘成连,马德功,等.欧洲苹果矮化自根砧苗木繁育技术[J].烟台果树,2010(2):30-31.

[5]邓丰产,马锋旺.苹果矮化自根砧嫁接苗繁育技术研究[J].园艺学报,2012,39(7):1353-1358.

[6]王荣,沈向,黄翠香,等.关于苹果砧木与自根砧快繁技术的综述[J].天津农业科学,2012,18(3):115-119.

[7]郭静,隗晓雯,王菲,等.SH40实生后代嫁接红富士苹果对其树体生长的影响[J].贵州农业科学,2013,40(10):146-148.

Last Update: 2014-12-12