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Cloning and Bioinformatic Analysis of the 3′-end of ERF Gene in Ziziphus jujuba(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年22期
Page:
93-97
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatic Analysis of the 3′-end of ERF Gene in Ziziphus jujuba
Author(s):
CHEN Ying-ying1; YUAN Zan1; ZHAO Jin2; LIU Meng-jun1
1.Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding,Hebei 071001;
2.College of Life Science,Agricultural University of Hebei,Baoding,Hebei 071001
Keywords:
Ziziphus jujuba; ZjERF; the 3′-end of ZjERF cloning; bioinformatic analysis
PACS:
Q 785；S 665
DOI:
-
Abstract:
Taking Ziziphus jujuba as material,the fragment of ERF gene contained 3′-end of ZjERF was first isolated by homologous gene cloning method on the basis of the homologous genes of Malus domestica,Arabidopsis thaliana and others in GenBank.The results showed that a 959 bp fragment was amplified.Conservation analysis of ZjERF showed that it had a typical zone of AP2/EREBP；similarity analysis showed that the protein had highly homologous to the ZjERF of Morus alba, Fagus sylvatica and Castanea sativa with homologous of 79%,78% and 77% respectively；Phylogenic analysis showed that ERF of species in jujube had a closer relationship with Theobroma cacao,Gossypium hirsutum,Gossypium barbadense and ZjERF blonged to B2 group of ERF family.Tertiary structure of ZjERF was speculated by Swiss Model program（http://au.expasy.org/tools/）.

References:

[1]Sakuma Y,Liu Q,Dubouzet J G,et al.DNA-Binding specificity of the ERFAP2 domain of Arabidopsis DREBs,transcription factors involved in dehydration-and cold-inducible gene expression[J].Biochemical and Biophysical Research Communications,2002,290(3):998-1009.

[2]Jofuku K D,Den Boer B G,van Montagu M,et al.Control of Arabidopsis flower and seed development by the homeotic gene APETALA2[J].The Plant Cell Online,1994,6(9):1211-1225.
[3]Ohme-Takagi M,Shinshi H.Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element[J].The Plant Cell Online,1995,7(2):173-182.
[4]Gu Y Q,Wildermuth M C,Chakravarthy S,et al.Tomato transcription factors Pti4,Pti5,and Pti6 activate defense responses when expressed in Arabidopsis[J].The Plant Cell Online,2002,14(4):817-831
[5]Zhang G,Chen M,Chen X,et al.Isolation and characterization of a novel EAR-motif-containing gene GmERF4 from soybean (Glycine max L.)[J].Molecular Biology Reports,2010,37(2):809-818.
[6]Okamuro J K,Caster B,Villarroel R,et al.The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis[J].Proceedings of the National Academy of Sciences,1997,94(13):7076-7081.
[7]Ohto M,Fischer R L,Goldberg R B,et al.Control of seed mass by APETALA2[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(8):3123-3128.
[8]Shen H,Wang Z Y.Expressional analysis of an EREBP transcription factor gene OsEBP-89 in rice[J].Acta Biochimica et Biophysica Sinica,2004,36(1):21-26.
[9]Zhang G,Chen M,Li L,et al.Overexpression of the soybean GmERF3 gene,an AP2/ERF type transcription factor for increased tolerances to salt,drought,and diseases in transgenic tobacco[J].Journal of Experimental Botany,2009,60(13):3781-3796.
[10]Zhou J,Zhang H,Yang Y,et al.Abscisic acid regulates TSRF1-mediated resistance to Ralstonia solanacearum by modifying the expression of GCC box-containing genes in tobacco[J].Journal of Experimental Botany,2008,59(3):645-652.
[11]Berrocal-Lobo M,Molina A,Solano R.Constitutive expression of ETHYLENE-RESPONSE-FACTOR1 in Arabidopsis confers resistance to several necrotrophic fungi[J].The Plant Journal:for Cell and Molecular Biology,2002,29(1):23-32.
[12]Zuo K J,Qin J,Zhao J Y,et al.Over-expression GbERF2 transcription factor in tobacco enhances brown spots disease resistance by activating expression of downstream genes[J].Gene,2007,391(1-2):80-90.
[13]Shin R,Park J M,An J M,et al.Ectopic expression of Tsi1 in transgenic hot pepper plants enhances host resistance to viral,bacterial,and oomycete pathogens [J].Molecular Plant-microbe Interactions,2002,15(10):983-989.
[14]周俊义,刘孟军,侯保林.枣疯病研究进展[J].果树科学,1998,15(4):354-359.
[15]刘孟军,赵锦,周俊义.枣疯病[M].北京:中国农业出版社,2010.
[16]王洋.植原体胁迫下枣SSH文库的构建及表达序列标签分析[D].石家庄：河北农业大学,2012.
[17]庄静.大白菜和甘蓝型油菜 AP2/ERF 家族转录因子的克隆与分析[D].南京：南京农业大学,2009.
[18]金慧,栾雨时.转录因子在植物抗病基因工程中的研究进展[J].中国生物工程杂志,2010,30(10)：99-104.
[19]Hao D,Ohme-Takagi M,Sarai A.Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant[J].Journal of Biological Chemistry,1998,273(41):26857-26861.
[20]Chen X,Guo Z.Tobacco OPBP1 enhances salt tolerance and disease resistance of transgenic rice[J].International Journal of Molecular Sciences,2008,9(12):2601-2613.
[21]陈林波,房超,王郁,等.茶树抗逆相关基因 ERF 的克隆与表达特性分析[J].茶叶科学,2011,31(1):53-58
[22]Park J M,Park C J,Lee S B,et al.Overexpression of the tobacco Tsil gene encoding an EREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco [J].Plant Cell,2001,13:1035-1046.
[23]秦捷,王武,左开井,等.AP2基因家族的起源和棉花AP2转录因子在抗病中的作用[J].棉花学报,2005,17(6):366-370.

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Last Update: 2014-12-12