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Mapping of Powdery Mildew Resistance Gene in Melon PMR5(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年21期
Page:
118-122
Research Field:
Publishing date:

Info

Title:
Mapping of Powdery Mildew Resistance Gene in Melon PMR5
Author(s):
WANG Xian-lei; NING Xue-fei; GAO Xing-wang; XIE Li-qiong; LI Guan
College of Life Science and Technology,Xinjiang University,Urumqi,Xinjiang 830046
Keywords:
melon; powdery mildew; gene; BSA; SSR
PACS:
Q 943.2
DOI:
-
Abstract:
Taking the powdery mildew resistance variety PMR5,susceptible variety Jiashi,F1,BC2 and BC2as materials,and the powdery mildew Podosphaera xanthii race 1 was inoculated.The method of resistance genetic analysis,Bulked Segregation Analysis (BSA) and Simple Sequence Repeat (SSR) markers were adopted to find the inheritance law of powdery mildew resistance,the markers linked to resistance gene,and then construct the genetic map the gene.The results showed that the resistant and susceptible separation ratio was 64∶27 in BC1,and 37∶27 in BC2,suggesting that PMR5 provided two dominant genes in BC1,and single dominant gene in BC2.A total of 11 SSR markers in LGII were found to linked powdery mildew resistance gene,and the gene was located in 104 113 bp between markers CMGA36 and SSR25208.

References:

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Last Update: 2014-12-01