|Table of Contents|

Primary Study on Tissue Culture and Physiology Character of Oncidium(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年21期
Page:
114-118
Research Field:
Publishing date:

Info

Title:
Primary Study on Tissue Culture and Physiology Character of Oncidium
Author(s):
CAO Yun-ying; HOU Hai-tao; LIU Yu-jie; ZHOU Rong; ZHAO Hua
College of Life Science,Nantong University,Nantong,Jiangsu 226019
Keywords:
Oncidium; tissue culture; protective enzyme activity; isozyme analysis
PACS:
S 682.31
DOI:
-
Abstract:
Taking Oncidium test-tube tissue as materials,the culture medium which was suitable for protocorm;bud seedling proliferation,making seedlings stronger and promoting the root growth of seedlings were screened;some physiological and biochemical indexes of growth process of plantlet regeneration and rooting were also monitored.The results showed that suitable proliferation culture medium for protocorm-like bodies (PLBs) was MS+1.0 mg/L 6-BA+0.4 mg/L NAA+1.0 g/L activated-charcoal,MS+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 g/L activated-carbon was suitable for adventitious bud multiplication.MS+0.5 mg/L 6-BA+1.0 mg/L 2,4-D+1.0 g/L activated-carbon was better for raising strong plants.1/2 MS+0.5 mg/L NAA+1.0 g/L casein hydrolysate was optimal for producing root.For the same sample,the activity and isozymes of superoxide dismutase and peroxidase because of different culture medium before transferring or the subculture time.SOD and POD activities of the differentiated seedlings sample which transferred from medium containing 0.1 mg/L NAA were higher than those transferred from 0.5 mg/L NAA.With the subculture times increased,its activities enhanced,the activity of sample from medium containing 0.5 mg/L 6-BA was higher than that from 2.0 mg/L 6-BA before transferring for PLBs,isozyme spectrum also proved this point.It suggested that medium containing 2.0 mg/L 6-BA were better than that containing 0.5 mg/L for PLBs proliferation,medium containing 0.5 mg/L NAA were better than that containing 0.1 mg/L for shoot multiplication.

References:

[1]段左俊,白旭华.文心兰的研究现状[J].热带林业,2006,34(1):24-26.
[2]郭志刚,五井正宪.嘉兰茎尖培养与块茎形成的研究[J].园艺学报,1998,25(2):179-183.
[3]曹孜义,刘国民.实用植物组织培养技术[M].兰州:甘肃科学技术出版社,1996：154-155.
[4]黄丽云,范海阔,周焕起,等.碳源浓度对文心兰组培生长的影响研究[J].江西农业学报,2010,22(3):64-66.
[5]刘晓荣,王碧青,朱根发.文心兰研究进展[J].亚热带植物科学,2007,36(3):85-90.
[6]陈心贻.文心兰的组织培养[J].植物生理学通讯,1989(6):49.
[7]崔广荣.文心兰组织培养及转基因研究进展[J].草业学报,2010,19(4):220-229.
[8]陈晓旋,李洪波,叶春海,等.文心兰类原球茎的诱导与增殖技术的优化[J].热带作物学报,2010,31(9):1464-1468.
[9]高丽,杨波,李洪林.叶艺春兰组培苗遗传稳定性的ISSR研究[J].亚热带植物科学,2007,36(2):13-14.
[10]刘玲梅,汤浩茹,刘娟.试管苗长期继代培养中的形态发生能力与遗传稳定性[J].生物技术通报,2008(5):22-27.
[11]张志良.植物生理学实验指导[M].北京:高等教育出版社,1990:154-155.
[12]曹云英,赵华.高温胁迫下油菜素内酯对水稻幼苗的保护作用[J].中国水稻科学,2007,21(5):525-529.
[13]罗广华,王爱国.植物SOD的凝胶电泳及活性显示[J].植物生理学通讯,1983(6):44-45.
[14]王海廷,黄永芬,汪清胤.番茄不同生育期和不同部位过氧化物同工酶分析[J].园艺学报,1981,8(4):29-35.
[15]刘鹏,杨玉爱.钼、硼对大豆叶片膜脂过氧化及体内保护系统的影响[J].植物学报,2000,42(5):461-466.

Memo

Memo:
-
Last Update: 2014-12-01