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Study on Tissue Culture and Rapid Propagation Technique of Different Genotype Strawberry(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年21期
Page:
110-113
Research Field:
Publishing date:

Info

Title:
Study on Tissue Culture and Rapid Propagation Technique of Different Genotype Strawberry
Author(s):
XUE Qi-qin; LI Mei-qin; LYU Jin-fu; PEI Hua-li; WANG Xing-cui; YANG Tian-hui
Biological Engineering Research and Development Center,Weifang University of Science and Technology,Shouguang,Shandong 262700
Keywords:
strawberry; genotype; hormone combination; activated carbon; tissue culture
PACS:
S 668.4
DOI:
-
Abstract:
Taking buds of strawberry‘Toyonoka’,‘Tochiotome’,‘Sweet Charlie’,‘Benihoppe’ and ‘Camarosa’as materials,the effects of different genotype strawberry,different hormone combination and active carbon on rapid propagation technique of strawberry were investigated.The results showed that the optimum medium for bud multiplication of five genotype strawberry was MS medium supplemented with 0.8 mg/L 6-BA and 0.5 mg/L NAA,MS medium supplemented with 1.2 mg/L 6-BA and 0.8 mg/L NAA,MS medium supplemented with 0.8 mg/L 6-BA and 0.8 mg/L NAA,MS medium supplemented with 0.8 mg/L 6-BA and 0.5 mg/L NAA,MS medium supplemented with 1.2 mg/L 6-BA and 0.5 mg/L NAA.And the propagation coefficient was 16.7,12.5,11.8,13.2 and 9.4 respectively.The most suitable rooting medium of ‘Toyonoka’,‘Sweet Charlie’ and ‘Benihoppe’was 1/2MS medium supplemented with 1.0 mg/L NAA.The most suitable rooting medium of ‘Tochiotome’ and ‘Camarosa’ was 1/2MS medium supplemented with 1.0 mg/L IBA.0.5% of activated carbon in the medium could increase the rooting of strawberry.Turfy soil and perlite(1∶1) was useful for transplantation,and the survival rate was 95%.

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Last Update: 2014-12-01