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Study on Extraction of Genome DNA in Gongshui Pomelo(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年21期
Page:
97-100
Research Field:
Publishing date:

Info

Title:
Study on Extraction of Genome DNA in Gongshui Pomelo
Author(s):
SHI Kai-ming; DENG Zhi-jun; FANG Xiang-liang
College of Forestry and Horticulture,Hubei Institute for Nationalities,Enshi,Hubei 445000
Keywords:
Gong-shui pomelo(Citrus grandis(L.) Osbeck); DNA; extraction
PACS:
S 602
DOI:
-
Abstract:
Taking Gongshui pemolo as test materials,water-saturated ether method，DNA phenol/chloroform method and basic CTAB method were used to extract DNA genome of Gongshui pomelo,the analysis and comparison was conducted from the yield of DNA samples,the appearance of DNA samples and the enzyme electrophoresis of DNA samples.The results showed that the water-saturated ether method proposed the best effect of appearance,which was pure white and fibrous for the frozen precipitation.The yield of genome DNA was the highest from the tender spike with phenol/chloroform treatment of young pomelo leaves,which obtained DNA concentration of 118 ng/μL.It indicated that the quality of DNA was more better with basic CTAB method and water-saturated ether method than phenol/chloroform method by EcoRⅠ.moreover,three methods all amplified clear bands of PCR by RAPD primers.

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Last Update: 2014-12-01