Study on the Optimization of Liquid Cultural Medium of Flammulina velutipes (PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the Optimization of Liquid Cultural Medium of Flammulina velutipes

Author(s):: GUO Xin-xin; FAN Shu-hua; WANG Yong-li; Department of Life Science，Zhoukou Normal University，Zhoukou,Henan 466001

Keywords:: Flammulina velutipes; liquid strain; culture medium; optimization

Abstract:: Taking Flammulina velutipes as material，the factors influencing mycelium growth were screened in order to determine the best liquid strain culture medium of Flammulina velutipes that produce intracellular polysacchride.The dry weight of the mycelia and the number of mycelium pellet were selected as the investigation index for determining the carbon source，nitrogen source，inorganic salt and vitamin.The fermentation test and the orthogonal test were used to analysis the four factors and to determine the optimum culture medium of liquid strain of F.velutipes.The results showed that the optimum carbon source for liquid culture of F.velutipes was soluble starch，the optimum nitrogen source was soybean flour，the optimum inorganic salt was KH₂PO₄，and the optimum vitamin was VB₂，and the optimum formula of culture medium was as follows：4% soluble starch，5% soybean flour，0. 05% KH₂PO₄，0. 0075% VB₂.

[1] 弓建国 . 食用菌栽培技术 [M]. 北京：化学工业出版社， 2011. 

[2]谢春芹,贾君,谢正林,等.金针菇菌渣栽培秀珍菇试验[J].北方园艺，2012(9)：170-172.

[3]蔡和晖,廖森泰,叶运寿,等.金针菇的化学成分、生物活性及加工研究进展[J].食品研究与开发，2008(11)：171-174.

[4]高淑敏.金针菇液体菌种培养液配方筛选试验[J].北方园艺，2011(12)：154-156.

[5]陈力力,吴新芬.金针菇液态发酵培养基的筛选[J].生物技术，2007，17(1)：73-75.

[6]马瑞霞,姚献华,魏志华.白色金针菇液体菌种生产技术研究[J].中国食用菌,2006,24(6)：16-17.

[7]姜宁,刘晓鹏,杨洪,等.金针菇液体培养基优化研究[J].安徽农业科学，2007，35(21)：6429-6430.

[8]杨子美,郭成金.正交设计法筛选槐耳菌丝体液体培养基的研究[J].天津师范大学学报，2009,29(3)：66-68.

[9]张桂春,李连梅,黄清荣,等.榆黄菇胞外多糖液体发酵培养基的优化[J].西北农业学报,2005,14(2)：155-157.

[10]秦俊哲,魏颖杰,陈合,等.金针菇液体菌种培养过程检测指标的研究[J].食用菌学报,2003，10(1)：12-16.

Last Update: 2014-08-10