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Research on Proliferation Culture Condition of Lilium leucanthum Baker Bulb(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年02期
Page:
112-116
Research Field:
Publishing date:

Info

Title:
Research on Proliferation Culture Condition of Lilium leucanthum Baker Bulb
Author(s):
TANG Ye-gang; CUI Wei
Wuhan Bioengineering Institute，Wuhan，Hubei 430415
Keywords:
Lilium leucanthum Baker; bulb; proliferation; solid culture; suspension culture; culture medium
PACS:
Q 949. 71+8
DOI:
-
Abstract:
Taking bulb sterile lines induced from the bulb of Lilium leucanthum Baker as experiment material，the bulb proliferation culture condition was studied from three aspects of disposition of explants，solid multiplication medium selection and liquid multiplication medium selection.The results showed that the proliferation effect of wounded bulb was significantly higher than the unwounded explants；the optimal solid multiplication medium was MS+6-BA 2. 0 mg/L+NAA 0. 3 mg/L+sucrose 30 g/L+agar 7 g/L，the average proliferation ratio was up to 7. 60；the best liquid multiplication medium was MS+TDZ 0. 1 mg/L+NAA 0. 3 mg/L+sucrose 30 g/L，the average multiplication ratio was up to 10. 86，and the average weight multiple was up to 6. 18；the effect of multiplication in the suspension culture medium of MS+6-BA 1. 5 mg/L+NAA 0. 4 mg/L+sucrose 30 g/L was significantly higher than the effect of multiplication in the solid medium.

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Last Update: 2014-08-10