Research on Tissue Culture of Regeneration System of the Mature Seeds of Bothriochloa ischaemum (PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Research on Tissue Culture of Regeneration System of the Mature Seeds of Bothriochloa ischaemum

Author(s):: YU Na¹; DONG Kuan-hu²; 1.Department of Landscape,Shanxi Forestry Vocational Technical College,Taiyuan,Shanxi 030009;
2.College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu,Shanxi 030801

Keywords:: Bothriochloa ischaemum; mature embryo; subculture; embryogenic callus; plant regeneration

Abstract:: Using the seeds of Bothriochloa ischaemum as the explants, with the method of tissue culture, the effect of different treatments of seeds on induction medium were studied.The results showed that hand stripping lemma for better processing method, during the callus induction phase, the culture medium of callus induction was MSB +2,4-D 2.0 mg/Ｌ and MSB+2,4-D 2.0 mg/Ｌ+KT 0.5 mg/L combined complete darkness culture that callus induction reached 70% and 75%; callus differentiation were improved evidence in the callus subculture medium.

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Last Update: 2014-08-10