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Cloning and Sequence Analysis of SSII Gene in Manihot esculenta Crantz(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年02期
Page:
99-103
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of SSII Gene in Manihot esculenta Crantz
Author(s):
ZENG Wen-dan1; 2; 3; LUO Xing-lu2; GUO Ya-jing2; YUAN Sheng-yong2; YANG Xin2
Manihot esculenta Crantz；soluble starch synthase isoform II gene；gene cloning；bioinformatic analysis
Keywords:
Taking Manihot esculenta Crantz cultivar SC124 as material; by RT-PCR technology; the whole sequece of SSII gene was firstly obtained.The results showed that the gene contained an 2 256 bp open reading frame (ORF) of SSII and encoding 751 amino acids.Comparaing to the published sequence(EF667961. 1); the homology of nucleotide sequence could shares 99%.However; the homology was low comaraing with other plants; about 77%~84%.The analysis of SSII polymorphism showed that there were alleles and paralogous gene in Manihot esculenta Crantz gene groups.
PACS:
S 889+.5
DOI:
-
Abstract:
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References:

[1]李永锋,赵光龙,张志强,等.国内木薯淀粉化学改性的研究进展[J].热带农业科学,2007,27（5）：64-72.

[2]Rolland-Sabaté A，Sánchez T，Buléon A,et al.Structural characterization of novel cassava starches with low and high-amylose contents in comparison with other commercial sources[J]．Food Hydrocolloids,2012,27(1):161-174.

[3]Zeeman S C,Kossmann J,Smith A M.Starch：its metabolism,evolution,and biotechnological modification in plants[J].Annu Rev Plant Biol,2010,61:209-234.

[4]谭彩霞,封超年,陈静,等.作物淀粉合成关键酶及其基因表达的研究进展[J].麦类作物学报,2008,28（5）：912-919.

[5]Commuri P D，Keeling P L.Chain-length specificities of maize starch synthase Ⅰ enzyme:studies of glucan affinity and catalytic properties[J].Plant J,2001,25(5):475-486.

[6]Edwards A，Fullon D C，Hylton C M，et al.A combined reduction in activity of starch synthase II and III of potato has novel effects on the starch of tubers[J].Plant J,1999,17(3):251-261.

[7]张付芸,陈伟霞,刘子渲,等.小麦SSⅡ-A基因片段的克隆及其RNAi表达载体的构建[J].中国农学通报,2011,27（7）：50-54.

[8]杨涛,张宁,栗亮,等.马铃薯可溶性淀粉合成酶SSⅢ基因克隆及生物信息学分析[J].分子植物育种,2009,7(3):545-549.

[9]Dian W,Jiang H,Ping W．Evolution and expression analysis of starch synthase III and IV in rice[J].J Exp Bot,2005,56(412):623-632.

[10]Ling K H,Loos S,Roslir,et al.In silico identification and characterization of a putative phosphatidylinositol 4-phosphate 5-kinase(PIP5K)gene inEimeria tenella?[J].In Silico Biology,2007,7(1):115-121.

[11]潘华清,何龙飞,何海旺.3飞机草总RNA提取方法比较研究[J].广西农业科学,2009，40（8）：957-960.

[12]陈丽珍,叶剑秋.我国木薯加工业的发展现状与展望[J].园艺与种苗,2011(3)：87-90.

[13]余春梅,陈佩度,季本华.小麦胚乳淀粉合成酶基因研究进展[J].麦类作物学报,2004,24（4）：123-128.

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Last Update: 2014-08-10