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Tissue Culture and Rapid Propagation of Dendranthema morifolium‘Qiandaiji’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年20期
Page:
105-108
Research Field:
Publishing date:

Info

Title:
Tissue Culture and Rapid Propagation of Dendranthema morifolium‘Qiandaiji’
Author(s):
SHI Min1; YANG Hong-yu2; ZHANG Guo-bin2; CHEN Qian1; YAN Bo1
1.College of Landscape Architecture,Southwest Forestry University,Kunming,Yunnan 650051;
2.College of Life Science and Technology,Kunming University,Kunming,Yunnan 650214
Keywords:
Chrysanthemum morifolium‘Qiandaiji’; tissue culture; hormone combination; optimizition
PACS:
S 682.21
DOI:
-
Abstract:
Taking leaf of Chrysanthemum morifolium ‘Qiandaiji’ as material,tissue culture conditions and media for callus induction,cluster buds propagation and rooting were studied in this article.The results showed that the proper medium of callus induction was SH+0.5 mg/L 6-BA+1.0 mg/L NAA and resulted in an induction efficiency of 100%.The cluster buds well grow in MS+2.0 mg/L 6-BA+0.2 mg/L NAA.And the appropriate rooting medium was 1/2MS+0.1 mg/L NAA.All three types of media were contained 30 g/L sucrose and 6.0 g/L agar with pH 5.8.The culture conditions were optimized to 2 500 lx light intensity,25℃ temperature and 12 h light/12 h dark circle.

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Memo

Memo:
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Last Update: 2014-11-21