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Study on Rapid Propagation of Cerasus avium ‘Hongdeng’ by Tissue Culture(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年20期
Page:
85-89
Research Field:
Publishing date:

Info

Title:
Study on Rapid Propagation of Cerasus avium ‘Hongdeng’ by Tissue Culture
Author(s):
LI Xiao-ling1; BIAN Zhen2; LU Xu-zhi1; ZHANG Jin-long1
1.School of Chemistry and Life Science,Changchun University of Technology,Changchun,Jilin 130012;
2.Library,Changchun University of Technology,Changchun,Jilin 130012
Keywords:
Cerasus avium ‘Hongdeng’; tissue culture; rapid propagation
PACS:
S 662.5
DOI:
-
Abstract:
Taking stem apex,axillary buds and young stems with axillary buds from the cold-tolerant plants of Cerasus avium ‘Hongdeng’ after acclimatization as explants,the protocol of rapid propagation by tissue culture was groped.The results showed that 90% of adventitious buds germinated on the primary medium contained MS basal salts and vitamins,6-BA 0.8 mg/L,NAA 0.02 mg/L,AC 0.5 g/L and 3% sucrose which was solidified with 0.7% agar(pH 5.8).After the shoots in vitro were subcultured for 25 days and so on the subculture medium contained 6-BA 0.6 mg/L,NAA 0.02 mg/L,GA3 0.6 mg/L,vitamin C 40 mg/L,the propagation coefficient by subculture could be up to 3.0.The shoots were rooted and grew stronger the 1/2 MS medium contained NAA 0.8 mg/L,vitamin C 40 mg/L and 1.5% sucrose,and 86.7% of shoots with strong roots survived after they were transplanted to soil.

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Last Update: 2014-11-21