|Table of Contents|

Gene Cloning and Sequence Analysis of psbA in Dryopteris fragrans(L.) Schott(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年19期
Page:
98-102
Research Field:
Publishing date:

Info

Title:
Gene Cloning and Sequence Analysis of psbA in Dryopteris fragrans(L.) Schott
Author(s):
GAO Rui1LIANG Yan-tao2CHANG Ying1
1.College of Life Science,Northeast Agricultural University,Harbin,Heilongjiang 150030;
2.College of Life Science,Daqing Normal University,Daqing,Heilongjiang 163712
Keywords:
Dryopteris fragrans(L.) SchottpsbARACEgene clone
PACS:
S 682.35
DOI:
-
Abstract:
Taking total RNA extraced from Dryopteris fragrans as material,and the RACE was employed to clone cDNA full length of psbA gene and complete bioinformatics analysis was conducted.The sequence had submitted to the GeneBank.The results showed that the full length of new cloned gene was 1 425 bp,containing an open reading frame of 1 062 bp,encoding 353 amino acids,there was high homology compared with other plant species.The new gene named DfpsbA,and its accession number was KJ728648.Successfully the result would lay a foundation for further research on photosynthesis mechanism,gene expression and regulation,as well as provide the basis for plant classification and evolution.

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Last Update: 2014-11-25