Taking ‘Lyudu fungicide No.1’ whose effective components is ‘Bacillus amyloliquefaciens Ba168’ as material,the effect of ‘Bacillus amyloliquefaciens Ba168’ on stem diameter,height,disease resistance and yield of the tomato were studied,to discuss the application effect of it on tomato growth of spring greenhouse in the north area.The results showed that ‘Bacillus amyloliquefaciens Ba168’ effectively promoted plant height,stem diameter,disease resistance and yield of tomato than that of CK.This research provided the practical reference for cultivation of the tomato in spring greenhouse.