|Table of Contents|

Cloning and Expression Analysis of Cinnamate 4-hydroxylase Gene from Prunus salicina and Isolation of Its Promoter(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年18期
Page:
122-127
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Cinnamate 4-hydroxylase Gene from Prunus salicina and Isolation of Its Promoter
Author(s):
WANG Yu-zhen 1; 2; CHEN Gui-xin 1; 2; ZHAO Li 1; 2; LYU Shi-heng1; PAN Dong-ming 1; 2; JIANG Cui-cui3
1.College of Horticulture，Fujian Agriculture and Forestry University，Fuzhou，Fujian 350002;
2.Institute of Storage Science and Technology of Horticultural Products，Fuzhou,Fujian 350002;
3.Fruit Research Institute，Fujian Academy of Agricultural Sciences，Fuzhou，Fujian 350013
Keywords:
Prunus salicina; Cinnamate 4-hydroxylase; promoter; real time PCR
PACS:
Q 943.2
DOI:
-
Abstract:
Taking the fruits of different development stages of Prunus salicina as materials，a normalized full-length cDNA library was built combining with two step method of reverse transcription and inhibit PCR technology,a full lenghth cDNA encoded Cinnamate 4-hydroxylase(C4H)(named PsC4H) was separated;the promoter was isolated by genome walking technology，changes of expression of the gene was detected by Real-time PCR at different development stages fruits.The results showed that the full-lenghth PsC4H was 1 772 bp with an open reading frame 1 515 bp and encoding 504 amino acids with a calculated molecular weight of 145 719.6 and theoretical pI of 4.94.Sequence homology analysis indicated that the amino acid sequence of PsC4H exhibit high homology to prunus plants.By PlantCare Software predict that in addition to TATA/CAAT-box，still contained some specific regulatory elements such as G-box，HSE and etc.Real time PCR experiment showed that the expression of PsC4H gene was down-up regulation through the entire developmental periods and was the highest at mature fruit stage.

References:

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Last Update: 2014-11-27