|Table of Contents|

Establishment of ISSR-PCR Reaction System for Anoectochilus roxburghii(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年18期
Page:
118-122
Research Field:
Publishing date:

Info

Title:
Establishment of ISSR-PCR Reaction System for Anoectochilus roxburghii
Author(s):
TANG Jun-rong1; TAN Xin-yi2; SUN Zheng-hai2; XIN Pei-yao1
1.Key Laboratory of Biodiversity Conservation in Southwest China,State Forestry Administration，Southwest Forestry University,Kunming，Yunnan 650224;
2.School of Horticulture and Gardening，Southwest Forestry University，Kunming,Yunnan 650224
Keywords:
Anoectochilus roxburghii; ISSR-PCR; orthogonal design
PACS:
Q 949.71+8.43
DOI:
-
Abstract:
The orthogonal design was used to optimize ISSR-PCR amplification system on Anoectochilus roxburghii in four levels of five factors (DNA template，Taq DNA polymerase，primer，Mg2+，and dNTP)respectively.The results showed that a suitable ISSR-PCR reaction system was established，namely 25 μL reaction system containing 40 ng DNA template，1.60 U Taq DNA polymerase，0.80 mmol/L primer，0.96 mmol/L dNTP，2.40 mmol/L Mg2+.In conclusion，this established ISSR-PCR reaction system provide an available and reliable method for genetic diversity analysis and germplasm clssificatin of Anoectochilus roxburghii.

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Last Update: 2014-11-27