Taking fine ornamental lily of Conca D’or as test material,tissue culture with its bulbus was studied.The results showed that the best sterilization modes and time were 75% ethanol 30 s+0.1% HgCl215 minutes,the contamination rate was 30.00%.The best combination of medium and hormone was MS+0.5 mg/L NAA+0.5 mg/L 6-BA,the average induced buds number was 5.87,and the average adventitious bud number was 2.87,the inside of the downward was the best ways of place on medium.