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Research on in vitro Tissue Culture of Pear Rootstocks OH×F51(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年18期
Page:
107-109
Research Field:
Publishing date:

Info

Title:
Research on in vitro Tissue Culture of Pear Rootstocks OH×F51
Author(s):
SONG Jian-kun; HUANG Man-na; WANG Ran
College of Horticulture，Qingdao Agricultural University，Qingdao，Shandong 266109
Keywords:
pear rootstock; OH×F51; shoot tips; leaves; in vitro tissue culture
PACS:
Q 813.1+2
DOI:
-
Abstract:
Taking pear rootstock OH×F51 as material,the shoot tips tissue culture and leaf callus induction of OH×F51 were studied,in order to gain the optimum way of in vitro culture.The results showed that the suitable medium for shoot tips culture was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+sugar 30 g/L+agar 6 g/L，with a higher rate of seedling.The optimum medium for leaf callus induction of OH×F51 was NN69+TDZ 3.0 mg/L+IBA 1.0 mg/L+sugar 30 g/L+agar 6 g/L.The placing manner of leaf had a certain influence on the callus induction.The leaves which abaxial surface touched the medium had a higher rate of callus.

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Memo

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Last Update: 2014-11-27