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Study on the Vitrification of Cotoneaster hjelmqvistii Plantlet in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年18期
Page:
103-106
Research Field:
Publishing date:

Info

Title:
Study on the Vitrification of Cotoneaster hjelmqvistii Plantlet in vitro
Author(s):
LU Xing-xia; CHAI Ci-jiang; ZHANG Ting; SHI Yan-shan; LUO Jian-xia
College of Horticulture and Landscape，Tianjin Agriculture College，Tianjin 300384
Keywords:
Cotoneaster hjelmqvistii Flinck and Turcz; tissue culture; vitrification phenomenon
PACS:
Q 949
DOI:
-
Abstract:
Taking Cotoneaster hjelmqvistii plantlet in vitro as materials，the effect of coverings of the vessels，6-BA concentrations (0~0.90 mg/L)，agar concentrations (6.5~8.5 g/L) and shoots culture temperature on its vitrification phenomenon were studied.The results showed that the vitrification rate was lower，and the propagation coefficient was higher when the Cotoneaster hjelmqvistii plantlet was cultured on MS basal salts containing 0.30 mg/L 6-BA and 6.5 g/L agar concentration，in 23~24℃ cultured temperature and in the vessels covering with cotton plug or sealing film.

References:

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Last Update: 2014-11-27