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System Optimization of TLC and HPLC Simultaneous Determination of Sophoridine and Matrine in Total Alkaloid of Sophora alopecuroides L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年18期
Page:
41-43
Research Field:
Publishing date:

Info

Title:
System Optimization of TLC and HPLC Simultaneous Determination of Sophoridine and Matrine in Total Alkaloid of Sophora alopecuroides L.
Author(s):
ZHOU Xing-chen1; LI Peng2; GU Pei-wen1
1.Agricultural College，Ningxia University，Yinchuan，Ningxia 750021;
2.Breeding Base of National Key Laboratory of Natural Gas Conversion，Province and Ministry Common Construction,Ningxia University，Yinchuan，Ningxia 750021
Keywords:
Sophora alopecuroides L.; alkaloid; TLC; HPLC
PACS:
Q 946.8
DOI:
-
Abstract:
Thin layer chromatography(TLC) was adopted，developing solvent was chloroform-methanol-ammonia=8∶2∶0.1;meanwhile，the chromatographic column of high performance liquid chromatography(HPLC) included Ultra IITM C18(5 μm×250 mm×4.6 mm) and mobile phase consisting of a mixture of 0.01 mol/L phosphate buffer-methanol (45∶55)，the detection wavelength was 216 nm，the flow rate was 1.0 mL/min;to establish the system optimization of TLC and HPLC methods for simultaneous separation and determination of alkaloids of Sophora alopecuroides L.The results showed that，the method had a good linearity and correlation coefficient:the sophoridine and matrine were 200～900 μg/mL.The mean recoveries (n=9) were 99.47% and 99.82% respectively.The experiment showed that，this method was simple，rapid and accurate assay for the determination of sophoridine and matrine in total alkaloid of Sophora alopecuroides L.，and suitable for quality control in the production of Sophora alopecuroides L.

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Last Update: 2014-11-26