Study on Extracellular Protease Activity Change During Fruitbody Growth of Cordyceps militaris(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年17期

Page:

148-150

Research Field:

Publishing date:

Info

Title:

Study on Extracellular Protease Activity Change During Fruitbody Growth of Cordyceps militaris

Author(s):

LIN Qun-ying¹; 2; WU Liang-liang¹; 2; ZHANG Feng-lu¹; WU Su-ling¹; SUN Xiao-ming¹; 3; XIANG Chun-rong³

1.Nanjing Institute for the Comprehensive Utilization of Wild Plants,Nanjing,Jiangsu 210042；

2.Jiangsu Hongfeng Fruit and Vegetable Co.Ltd.,Suqian,Jiangsu 223700;

3.Zhengdong Ecological Agriculture Development Center of Zhenjiang City Dantu District,Zhenjiang,Jiangsu 212000

Keywords:

Cordyceps militaris; fruiting body cultivation; extracellular proteases

PACS:

S567.35

DOI:

-

Abstract:

Taking Cordyceps militaris as materials,the fresh weight of fruit body,pH value in medium,and the activity of extracellular acid protease,neutral protease and alkaline protease during growth of Cordyceps militaris were investigated.The results showed that the activity of acid protease could be affected obviously by nitrogen but not the profile.The highest activity of 112 U/g was promoted to 213 U/g by nitrogen supplement.Both activities and profiles of neutral and alkaline proteases were affected by nitrogen supplement.The highest activity of neutral protease was recorded on the 30th day with 10.2 U/g.And no obvious maximum was detected on substrate without nitrogen supplement.Alkaline protease activity began to rise on the 10th day and reached to the highest (10 U/g) at the 50th day on nitrogen-supplemented substrate.And alkaline protease activity started to rise only on the 50th day and got to highest (6.5 U/g) at the end of the culture.The findings could be helpful for the nutritional physiology researches of Cordyceps militaris.

References:

［1］Das S K,Masuda M,Sakurai A,et al.Medicinal uses of the mushroom Cordyceps militaris:Current state and prospects［J］.Fitoterapia,2010,81(8):961-968.

Memo

Memo:

-

Common functions