|Table of Contents|

Study on Elimination and Recrudescence of Apple Latent Virus by Alternating Heat Therapy in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年17期
Page:
130-134
Research Field:
Publishing date:

Info

Title:
Study on Elimination and Recrudescence of Apple Latent Virus by Alternating Heat Therapy in vitro
Author(s):
GUO Chao; WU Ran; SHAO Jian-zhu; SUN Jian-she; SHI Xiao-xin
College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071000
Keywords:
apple latent virus; elimination; recrudescence
PACS:
S661.1
DOI:
-
Abstract:
Taking four apple rootstocks and cultivars（‘Malus robusta Rehd.’,‘Malus xiaojinensis.’,‘Huahong’,‘JAZZ’）infected with ASPV,ACLSV and ASGV as materials,alternating heat therapies in combination with shoot tip culture were used to eliminate apple viruses,the RT-PCR system was used to detect and real-time track viruses in virus-free plantlets,the responses to alternating heat therapy in combination with shoot tip culture on latent virus elimination and suitable detection time in different cultivars and rootstocks vitro were studied.The results showed that under different heat treatment conditions,rates of virus-elimination were different.Rates of virus-elimination under same treatment differed from plant to plant.‘Malus xiaojinensis’ listed the top followed by ‘JAZZ’ and ‘Malus robusta Rehd.’,while ‘Huahong’ ranked number three.However,viruses were always inactivated in the same order：first ASPV,followed by ACLSV,last ASGV.Additionally,among the four detections,the detection rate of ASGV was highest.About 180 days,the detection rate of all latent virus reached a peak.Until 220 days,ASGV,ASPV and ACLSV were detected completely.And the detection sequence was that ASGV was detected first,ACLSV followed,ASPV was the latest.So it was necessary to examine more than once in apple virus-free seedlings.

References:

［1］王国平.果树病毒检测与脱除技术的研究进展［J］.华中农业大学学报,2004(6):685-691.

［2］陈红霞.我国苹果主产区潜隐性病毒侵染特征研究［D］.保定：河北农业大学,2012.
［3］程玉琴,韩振海,徐雪峰.苹果病毒及其脱毒检测技术研究进展［J］.中国农学通报,2003,19(1):72-73,96.
［4］王国平.苹果病毒病防治［M］.北京:金盾出版社,1994.
［5］王焕玉,刘福昌,薛光荣,等.苹果母本树脱除病毒的研究［J］.中国果树,1991(4):15-17,35.
［6］董淑英,位绍文,孙静,等.苹果脱毒方法的比较研究［J］.莱阳农学院学报,2002,19(2):112-115.
［7］程玉琴,徐践,张勇,等.苹果品种和砧木组培苗对热处理脱毒的反应［J］.植物保护科学,2003,19(6):216-219.
［8］王壮伟.苹果潜隐性病毒的检测与脱除技术研究［D］.南京:南京农业大学,2006.
［9］胡晶.变温热处理结合茎尖培养脱除沙梨离体植株潜隐病毒研究［D］.武汉:华中农业大学,2011.
［10］曹晓凤,邵建柱,孙建设.几种提取液对苹果成熟叶片总RNA提取质量的影响［J］.安徽农业科学,2011,39(5):2570-2571,2607.
［11］陈红霞,邵建柱,孙建设,等.两步多重RT-PCR快速检测苹果潜隐性病毒［J］.果树学报,2012,29(4):695-701.
［12］王利平.热处理和化学处理对梨离体植物中病毒的影响［D］.武汉:华中农业大学,2006.
［13］谭荣荣.物理及化学处理脱除砂梨潜隐病毒研究［D］.武汉:华中农业大学,2006.
［14］晏娜,孙建设,邵建柱.22个苹果品种和砧木试管苗对热处理脱毒效应的研究［J］.河北农业大学学报,2009,32(5):40-44.
［15］Cropley R.Comparison of some apple latent viruses［J］.Annals of applied Biology，1968,61：361-367
［16］洪霓,王国平,于济民,等.苹果茎沟病毒的分离纯化及血清学检测［J］.中国农业科学,1997,30(5):6-12.
［17］吴雅琴,王文慧,章德明,等.苹果茎痘病毒病原学研究初报［J］.河北果树,2000(4):7,19.［18］German S,Candresse T,Le Gall O,et al.Analysis of the dsRNAs of apple chlorotic leaf spot virus［J］.Journal of General Virology，1992,73：767-773.
［19］姜中武,张振英,都韶英.苹果褪绿叶斑病毒的特性与为害［J］.烟台果树,1995（4）:23-24.
［20］赵霜,李青,时颂.不同处理方法对菊花‘神马’脱除病毒的影响［J］.东北林业大学学报,2013,41(3):103-106.
［21］高慧卿,樊兰瑛,王秀红,等.茎尖培养及热处理技术在百合脱毒中的应用研究［J］.山西农业大学学报,2010,30(6):528-532.
［22］董雅凤,张尊平,张少瑜,等.苹果和梨树茎尖培养结合热处理脱病毒研究［J］.北方果树,2002(2):9-11.
［23］晏娜.化学处理和热处理脱除苹果潜隐性病毒的研究［D］.保定:河北农业大学,2009.
［24］江峰,孙振华.苹果试管苗热处理脱毒法的研究［J］.落叶果树,2000(1):4.

Memo

Memo:
-
Last Update: 2014-09-26