Adventitious Bud Induction and Proliferation Studies in Tissue Culture of Lonicera caerulea‘Lanxin’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Adventitious Bud Induction and Proliferation Studies in Tissue Culture of Lonicera caerulea‘Lanxin’

Author(s):: LIANG Li-dong; LI Ming-wen; ZHU Li-guo; Heihe Academy of Forestry Sciences,Heihe,Heilongjiang 164300

Abstract:: Taking sterile stem buds of Lonicera caerulea ‘Lanxin’ as material,the effect of different concentrations of cytokinin 6-BA and different concentrations of auxin NAA and IBA on induction and proliferation of adventitious buds were studied.The results showed that there were a significant impact on adventitious bud induction rate and proliferation coefficient in different concentrations of cytokinin 6-BA and different concentrations of auxin NAA and IBA；induction rate and proliferation coefficient of adventitious buds were significantly improved in different concentrations of cytokinin 6-BA,effect on induction and proliferation coefficient of adventitious buds in different concentrations of auxin NAA were better than that in different concentrations of auxin IBA；comprehensive analysis showed that adventitious buds induction rate of Lonicera caerulea ‘Lanxin’ could reach 90%,proliferation coefficient could reach 1.58,further adventitious buds were the robust growth in the case of WPM medium added 6-BA of 0.8 mg/L concentrations and NAA of 0.15 mg/L concentrations.

［1］沈海龙.植物组织培养［M］.北京:中国林业出版社,2005.

［2］赵越,霍俊伟,王丽娟.蓝靛果的组织培养及植株再生［J］.植物生理通讯,2003,39(5):468.

［3］李桂君,李艳霞,卢慧颖,等.俄罗斯耐寒蓝靛果忍冬组织培养技术研究［J］.林业科技,2012,37(4):4-5.

［4］田新华,吴捷,张建瑛,等.蓝靛果忍冬组织培养研究［J］.林业科技,2012,37(6):7-9.

［5］梁琦兰,张启昌,杨振国,等.蓝靛果忍冬芽体组织培养技术研究［J］.北华大学学报,2006,7(6):549-551.

［6］张启昌,梁琦兰,夏新莉,等.蓝靛果忍冬茎段离体培养与植株再生［J］.北京林业大学学报,2010,32(4):126-130.

［7］张法勇,刘向东,高秀丽.木本植物组织培养器官发生植株再生研究进展［J］.河北林果研究,2005,20(3):234-238.

Last Update: 2014-09-26