|Table of Contents|

Cloning and Transformation of NCED4 Gene from Arabidopsis thaliana(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年17期
Page:
105-110
Research Field:
Publishing date:

Info

Title:
Cloning and Transformation of NCED4 Gene from Arabidopsis thaliana
Author(s):
GUO Tao１; ２; CHAO Yue-hui2; YANG Qing-chuan2; JIN Hong1; ZHANG Tie-jun2; KANG Jun-mei2
1.Collefe of Ecology and Environment,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia 010019；
2. Beijing Institute of Animal Husbandry and Veterinary,Chinese Academy of Agricultural Sciences,Beijing 100193
Keywords:
Arabidopsis thaliana; NCED4; abscisic acid; tranformation
PACS:
Q943.2
DOI:
-
Abstract:
Taking Arabidopsis thaliana as material,a gene which was named as NCED4was cloned by PCR technique.NCED4 gene was inserted into plasmid and resulting a plant expressing vector pBI-NCED4 with DNA recombination technology.Transgenic Arabidopsis plants with kanamycin resistance were obtained finally,and this were the basis of researching that the effect of NCED4 gene overexpression on plant.The results showed that，PCR and Real Time RT-PCR analysis of 4 kanamycin resistant plants showed that the target gene was inserted into the genomes of the Arabidopsis thaliana and NCED4 gene was over-expressed in transgenic Arabidopsis successfully;the expression levels of NCED4 genes were changed obviously when exogenous hormones were added,and these results showed that NCED4 gene may play an important role in hormone biosynthesis and regulation.

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Last Update: 2014-09-26