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Analyse of Peroxidase and Superoxide Dismutase Isozymes During Seed Germination on Handeliodendron bodinieri (Levl.) Rehd.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年17期
Page:
102-105
Research Field:
Publishing date:

Info

Title:
Analyse of Peroxidase and Superoxide Dismutase Isozymes During Seed Germination on Handeliodendron bodinieri (Levl.) Rehd.
Author(s):
LI Lei1; LI Xue-ping2; GUO Song1; 3; PENG Jian1; LI Zai-liu1; 4
1.College of Forestry,Guangxi University,Nanning,Guangxi 530004;
2.College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003；
3.Jiangsu Key Laboratory of Forestry Ecological Engineering,Nanjing Forestry University,Nanjing,Jiangsu 210037;
4.College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan,Hubei 430070
Keywords:
Handeliodendron bodinieri (Lévl.) Rehd.; peroxidase isozymes; superoxide dismutase isozymes; seed germination
PACS:
Q949.755.5
DOI:
-
Abstract:
Taking the seeds of Handeliodendron bodinieri (Lévl.) Rehd.as materials,vertical slab polyacrylamide gel electrophoresis method was used to study the dynamic change of peroxidase(POD) and superoxide dismutase(SOD) during the germination of Handeliodendron bodinieri seeds which had removed the seed coat.The results showed that,in the early seed germination period (0~2 d),the dormant state of seeds was broken and absorbed water to synthesize a few POD and SOD enzymes.In the middle (3~5 d),seeds began to germinate,radicle elongated,new bands of SOD and POD isozymes were synthesized,content increased rapidly,marked the seeds begin to germinate.In the later stage(6~9 d),radicle continued elongating,cotyledon unfolded gradually,embryo began to grew,SOD and POD isozymes content decreased,but the type of each isozyme unabated,new enzyme was synthesized.The changes of those isozymes could be used as an important sign in each stage of Handeliodendron bodinieri seed germination.

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Last Update: 2014-09-26