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Effect of Three Antioxidants on Browning of Explant and Callus Induction Rate of Miscanthus lutarioriparius(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年17期
Page:
93-96
Research Field:
Publishing date:

Info

Title:
Effect of Three Antioxidants on Browning of Explant and Callus Induction Rate of Miscanthus lutarioriparius
Author(s):
XIA Ya-nan; JIANG Jian-xiong; YI Zi-li; LIU Qing-bo; HUANG Hong-mei; CHEN Zhi-yong
Research Institute of Miscanthus,Hunan Agricultural University,Changsha,Hunan 410128
Keywords:
Miscanthus lutarioriparius; tissue culture; browning; polyvinylpyrrolidone; vitamin C; citric acid
PACS:
Q813.1+2
DOI:
-
Abstract:
Taking the immature inflorescences of M.Lutarioriparius as explants and MS medium with polyvinylpyrrolidone (PVP),vitamin C or citric acid (CA) were used as medium in this research,the effect of 3 antioxidants to inhibit browning of explants and on callus induction rate of Miscanthus lutarioriparius were studied.The results showed that treating with PVP,vitamin C and CA could improve callus induction rate and reduce the browning of explants in a certain degree.Compared with the control whose callus induction rate were 35.83%,adding PVP,vitamin C or CA in the medium increased the induction rate to 55.42%,50.42% and 124.00% respectivly；and adding CA made highly significant difference (P<0.01).CA was the best anti-browning agent of immature inflorescences culture of M.lutarioriparius,and 0.2 g/L CA could significantly increase the callus induction rate by 2.46 times.

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Last Update: 2014-09-26