Selection for a Hericium erinaceus Mutant Strain in the Way of Protoplasts Being Exposed to the UV Radiation(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Selection for a Hericium erinaceus Mutant Strain in the Way of Protoplasts Being Exposed to the UV Radiation

Author(s):: WANG Qian¹; MA Xu-guang¹; LIU Min¹; YANG Shu-qin²; 1.College of Life Science,Hebei University,Baoding,Hebei 071002;
2.Special Care Hospital of Hebei Province,Shijiazhuang,Hebei 050051)

Keywords:: Hericium erinaceus; protoplasts; exposition to UV radiation; esterase isozyme profiles

Abstract:: Use Hericium erinaceus He001 that stored in Institute of Medicinal Fungi of Heibei University,273 strains were regenerated in the process of protoplasts of He001 being exposed to the UV radiation.14 strains were filtered out based on the mycelial antagonistic symbols,and 8 strains,of which mycelial growth rates were higher than the original,were chosen to detect their esterase isozyme to confirm whether they were genetically different from the original.The results showed that there were four bands of esterase isozyme located in the range of the migration rate between 0.41 and 0.59 in all.The association coefficient between H035 and He001 was 0.33,which was the smallest and meant H035 was genetically different from He001.Besides,the mycelia growth rate of strain H035 was higher than He001,and the difference was extremely significant.On the basis of all facts above,H035 was confirmed to be a mutant strain.

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Last Update: 2014-09-26