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Study of the Genetic Relationships of Main Lentinula edodes Cultispecies in Qinba Mountainous Area(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年16期
Page:
140-143
Research Field:
Publishing date:

Info

Title:
Study of the Genetic Relationships of Main Lentinula edodes Cultispecies in Qinba Mountainous Area
Author(s):
ZHAO Na-na1; CHEN Wen-qiang1; 2; DENG Bai-wan1; 2; LAN A-feng1; 2; XIE Xiu-chao1; 2; WANG Jin1; QIAO Yan-ming1; SU Chen-xi1
1.School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723000;
2.Shaanxi Engineering Research Center of Edible and Medicated Fungi,Hanzhong,Shaanxi 723000
Keywords:
Lentinula edodes; strain; morphological characteristics; ITS sequence; kinship.
PACS:
S646
DOI:
-
Abstract:
Using ten main Lentinula edodes cultivated in Qinba mountainous area as malerals,the growth rate,mycelium morphology of they were analyzed by tissue cultured method.Meanwhile,CTAB method was used to extract genomic DNA from the mycelium,amplifying and sequencing PCR products,and ITS analysis of Lentinula edode and phylogenetic tree were conducted.The results showed that ‘Lentinula edodes 808’ and ‘Lentinula edodes 507’ owned growth advantage and distant relationship,as dominant strains protoplast formation,fusion and regeneration.Our findings established a solid foundation to determine the genetic kinship of cultivated species and the cultivation of new varieties in Qinba mountainous area.

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Last Update: 2014-09-26