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Study on Identification and Biological Characteristics of Caused Agent of Leaf Perforation on Blueberry(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年16期
Page:
123-126,127
Research Field:
Publishing date:

Info

Title:
Study on Identification and Biological Characteristics of Caused Agent of Leaf Perforation on Blueberry
Author(s):
YAN Xue-rui; ZHOU Yuan; ZHAO Rui-jie; DAI Han-ping; FU Jun-fan
College of Plant Protection,Shenyang Agricultural University,Shenyang,Liaoning 110866
Keywords:
blueberry; leaf spot; Alternaria alternata; identification; ITS; biological characteristics
PACS:
S663.9
DOI:
-
Abstract:
Taking sample of leaf perforation identified in blueberry production areas as test material,based on Koch’s postulates,morphological characters,rDNA-ITS and EF-1α sequences blust and biological characters method,the pathogenic bacteria,classification status of pathogenic bacteria,pathogen mycelia growth and spore germination under different culture conditions were studied.The results showed that the pathogen was identified as Alternaria alternata,the mycelia grew best on PDA media,under 30℃,pH 5,dextrose and glycine were the best carbon and nitrogen source respectively.The spore germination condition was 30℃,pH 5,2% glycine and 2% dextrose suspension.the mycelia died under 56℃ for 10 min.

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Last Update: 2014-09-26